9. Explain about Detoxification by conjugation.
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- 8. To observe bacteriophage in a lab, you would plate dilutions of the phage onto lawns of bacte- ria and observe the formation of after incubation. Hint: Specific name only.9, Why are new strains of Influenza more problematic?3. Label the following elements of the figure below: lysogenic phage, lysogenic cycle, lytic cycle, prophage, phage DNA, bacterial chromosome, and self assembly.
- 6. During penetration of E. coli by a bacteriophage lysozyme is used to allow entry of the bacteriophage capsid and nucleic acid the tail acts as a hypodermic needle, injecting the bacteriophage DNA into the cell the tail fibers would bore a hole in the cell wall the virus would also bind to Sa e. E. coli would attempt to protect itself by synthesizing enzymes that degrade bacteriophage RNA 7. Penicillin-binding proteins can be bound by beta-lactam drugs in the cell b. alter the beta-lactam ring c. are involved in cell wall synthesis d. are synthesized by eukaryotic cells .a&c 8. As we discussed in class, basophils are white blood cells, and one of their roles in the host immune response is to release histamine. Based on this information, please answer the following question. Which of the following processes or cells will be affected as a result of your patient having basopenia, which is a low number of basophils? The production of C3A The production of CSA The production of pus The…1. Explain how scientists could have identified the T toxin 2. Name one enzyme which would have been used during cloning at stage 2.20. P2 and P4 are bacteriophages of E. coli. They have the following properties: (1) When P2 phage infects a bacterium, the bacterium eventually bursts, yielding about 100 P2 progeny. (2) When P4 infects a bacterium, the bacterium survives because P4 is a defective phage. (3) When P2 phage and P4 phage coinfect the same bacterium, lysis of the bacterium yields 100 P4 progeny and no P2 progeny, because the presence of P4 inhibits the growth of P2. Suppose 3 x 108 P2 and 2 x 108 P4 are added to 108 bacterial cells. How many bacterial cells will produce P2 progeny? A. 1.28 x 107 В. 6.75 х 105 С. 5.00 х 106 D. 2.56 x 107 E. 3.23 x 104
- 1. List and describe the 6 steps in the replication of T4 phage in E. coli24. Which of the following antibiotics would you use to stop the growth of mycoplasma. A) Penicillin, an antibiotic that blocks cell wall biosynthesis. B) Tetracycline, an antibiotic that inhibits bacterial protein synthesis by blocking the function of ribosomes.6- The following diagram shows the life cycle of the lytic virus, the symbol that indicates the stage of bacterial cell explosion and decomposition:
- 1. In terms of Spo11, what does it mean if twice as many genetic sequence reads map to one location than another? Explain. 2. What does it mean if genetic reads map in different locations in difgerent strains? Explain in terms of recombination hotspots.12. What is the difference between pathogenic and non-pathogenic E. coli in relation to the human host?11. A phage cross is made by doubly infecting the host bacterial cell with parental phages: mtg X mīgt. What are the expected recombinant genotypes of the progeny phage?
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