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Dilution results in a decrease in the concentration of a substance in a mixture. A stock solution is…

Answered: 7. You have a 200 mg/mL stock solution…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).

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7. You have a 200 mg/mL stock solution of protein. To make 20mL of 5mg/mL solution, add of stock and of water for a final volume of mL. Question # 7 mL of stock mL mL of water mL mL final volume mL