7 A-C

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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7 A-C
102
rIIB+
D1
Lab 6: Mapping the rII Locus of Bacteriophage T4
7) Hypothetical situation: In the bacteriophage T4, each of 5 different independently arising rII point
mutations (designated as V, W, X, Y and Z) produce no recombinants when co-infected (in a permissive
host bacteria) with phage carrying a deletion of the entire cistron B of the rII region. (This deletion does
not extend into cistron A)
a) What does a lack of recombinant progeny indicate about the position of the mutations?
D2
D3
D4
D5
b) The following deletion map shows the rIIB locus in the bacteriophage T4. Five deletion mutant
strains designated as D1, D2, D3, D4 and D5 have been mapped to the rIls region.
The outcome of a series of co-infections is summarized in the following table.
Deletion Mutants
Point
Mutants
V
W
X
Y
D1
+
+
-
D2
+
+
D3
-
+
+
D4
-
+
-
+
+
Z
+
+
+
Clearly place the five point mutants on the wild type rIIB* region above using vertical arrows."
c) Assuming mutants V and Y are different, how could you determine their precise locatic
D5
+
+
+
+
+
Lab
de
Every
from the
repair U
cloudy d
effect of
become
1. To-
a)
b)
SANA
1. TH
The
shorte
Some
fairly lo
this lig
absorb
moder
excess
U
adjac
kinke
resu
oppo
is th
of p
of b
acc
Transcribed Image Text:102 rIIB+ D1 Lab 6: Mapping the rII Locus of Bacteriophage T4 7) Hypothetical situation: In the bacteriophage T4, each of 5 different independently arising rII point mutations (designated as V, W, X, Y and Z) produce no recombinants when co-infected (in a permissive host bacteria) with phage carrying a deletion of the entire cistron B of the rII region. (This deletion does not extend into cistron A) a) What does a lack of recombinant progeny indicate about the position of the mutations? D2 D3 D4 D5 b) The following deletion map shows the rIIB locus in the bacteriophage T4. Five deletion mutant strains designated as D1, D2, D3, D4 and D5 have been mapped to the rIls region. The outcome of a series of co-infections is summarized in the following table. Deletion Mutants Point Mutants V W X Y D1 + + - D2 + + D3 - + + D4 - + - + + Z + + + Clearly place the five point mutants on the wild type rIIB* region above using vertical arrows." c) Assuming mutants V and Y are different, how could you determine their precise locatic D5 + + + + + Lab de Every from the repair U cloudy d effect of become 1. To- a) b) SANA 1. TH The shorte Some fairly lo this lig absorb moder excess U adjac kinke resu oppo is th of p of b acc
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