625 nm, which can be used for the spectrophotometric determination of ammonia. ocr -OH • NH, indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake water is mixed with 5 mL of phenol solution and 2 ml of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL. of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 × 104 M ammonia solution, and diluted to 25.0 mlL. Sample C is a reagent blank. It contains 10.0 ml. of distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) A 0.374 B 0.621 0.045 What is the molar absorptivity (e) of the indophenol product? M'cm What is the concentration of ammonia in the lake water? [NH,Jhtc water M

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Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at
625 nm, which can be used for the spectrophotometric determination of ammonia.
ocr
-OH • NH,
indophenol anion
To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake
water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric
flask. In sample B, 10.0 mL of lake water is mixed with 5 mL. of phenol solution, 2 mL of sodium hypochlorite solution, and
2.50 mL of a 5.50 x 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of
distilled water, 5 mL of phenol solution, and 2 ml. of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the
three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table.
Sample Absorbance (625 nm)
A
0.374
В
0.621
0.045
What is the molar absorptivity (c) of the indophenol product?
e =
M'cm
What is the concentration of ammonia in the lake water?
(NH, lake water
M
Transcribed Image Text:Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which can be used for the spectrophotometric determination of ammonia. ocr -OH • NH, indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL. of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 x 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 ml. of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) A 0.374 В 0.621 0.045 What is the molar absorptivity (c) of the indophenol product? e = M'cm What is the concentration of ammonia in the lake water? (NH, lake water M
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