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6. You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul, how would you create 500µl of a 15ug/ul RNA solution?

6. You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul, how would you create 500µl of a 15ug/ul RNA solution?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul, how would you create 500ul of a 15ug/ul RNA solution? You are given a 10X PCR buffer. You will be setting up a PCR that will have a final total volume of 40 1. How much 10X PCR buffer will you put in the reaction, so that the reaction has 1X buffer? 7. from 6.
Transcribed Image Text:You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul, how would you create 500ul of a 15ug/ul RNA solution? You are given a 10X PCR buffer. You will be setting up a PCR that will have a final total volume of 40 1. How much 10X PCR buffer will you put in the reaction, so that the reaction has 1X buffer? 7. from 6.
Making Solutions Practice Problems Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of vour calculations, In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurafely pipette is Ipl. (CaCly: Molecular weight - 111.0 - NaCI: Molecular weight-58.44)
Transcribed Image Text:Making Solutions Practice Problems Part I. For each of the following four questions: calculate and describe how the requested solution would be made. Please show all of vour calculations, In all cases the diluent or solvent will be water. Also, assume the smallest volume you can accurafely pipette is Ipl. (CaCly: Molecular weight - 111.0 - NaCI: Molecular weight-58.44)
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