6/ The critical process in microscopic preparation of samples is * Mounting O Labelling Staining 8/ Safranin is an acidic pigment. O True O False 17/The ratio of Interphase form Life cycle of cell * 90% 80% 60%
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- schema __ illustrates simple segragation. shema__illustrates how interpersion is violated during oven incubations schema___has randomization but poor interspersion6) You want to determine the amount of cells in a culture. You dilute the suspension to 10-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?Plateau Phase Death Lag Phase Exponential Growth Phase Phase Fen ve cels Many ive cets Dead cells 10 15 20 25 30 35 40 45 Hours Consider the above figure which depicts the growth curve of microorganisms such as Saccharomyces cerevisiae. Now, reflect upon the components which make up the growth medium used to cultivate yeast. Whilst the growth medium was being made, the component which provides a source of energy for yeast was omitted. VWhat component was left out? What effect will this have on the growth curve phases and the rate at which they occur, especially in regard to the 'Exponential Growth' phase of the yeast? Loganmm/10of Vahie Cels
- 8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 ΣI U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III45years old male had been working e his garden when he scratched the knuckle of his right aram during digging in ground with a spade,he immediately washed the area but noticed that the knuckle became red,warm to the touch and swollen.he went to hospital,the doctor ordered a hram stain and culture on the pus extracted from the wound. Which culture grew?
- I Why does't a negative stain colorize the cells in the smear?Im 100 4. You have a culture of yeast that is at a density of 8x10' cells/mL. You dilute the sample 1:10, then 1:1,000 again, and finally you dilute the sample an additional 1:5. You add 0.1 mL of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many colony- forming units do you expect to count on your pour plate the next day?12 12 Choose the letter of correct answer and write it on the paper! How can we destroy the harmful Aspergillus sp. (fungi) cell (isosomotic concentration in cell: 0,8 M NaCl) in the most easily way that can be seen on the picture? A)Cell is put into 0,2 M NaCl solution B)Cell is put into 0,4 M NaCl solution C)Cell is put into 0,9 M NACI solution 2021.10.01. D)Cell is put into 1,6 M NaCl solution 91.5% A Rodlet Layer • RodA, RodB Dispersal Plasma Membrane Lactoferrin Iron Zinc Nitrogen Host Proteins Introduction to Cell Biology TBBE5000 EN 2. lesson. 2020/2021 1st semester Secreted Proteases GPI-anchored proteins Catalase, SOD Slalic Acid Residues Siderophores, Zn Transport Nitrogen Uptake Call Wall Mycotoxins Cell wall •a-glucan B-glucan Chitin • Galactomannan Melanin ROI Pentraxin-3 DC-SIGN Fibronectin
- ery t ess Which method of counting cells would you use for each of these situations? Viable Cell Count Optical Density measurement Direct cell count [Choose ] [Choose ] ✓ [Choose ] You are attempting to quantify the ability of cleaning agents to kill bacteria in a liquid culture You are attempting to determine the doubling time of Escherichia coli in various liquid media You are attempting to quantify the ratio of contaminating rod-shaped cells in your culture of spherical Staphylococcu6 (a). From the following diagram, what would the name of the newly created dissacharride be? TYPE ANSWER IN LOWER CASE * CHOH CHOH HO OH OH, HO OH он OH Онwwwwww The total number of cells in a culture is counted using dye trypan blue and is found to be 3.8 x 106 cells/mL. The culture is diluted 1:22 and then 200μl seeded per well into a 96 well plate. What is the final cell density per well? ssibility: Good to go MacBook Air