5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilutie scheme and include the overall dilution in each tube. From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After incubation, you count the plates and get the following results: What was the number of organisms/ml in the initial culture?
5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilutie scheme and include the overall dilution in each tube. From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After incubation, you count the plates and get the following results: What was the number of organisms/ml in the initial culture?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilution
scheme and include the overall dilution in each tube.
From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After
incubation, you count the plates and get the following results:
What was the number of organisms/ml in the initial culture?
Final dilution
No. of
colonies 0.1
No of
colonies 1
ml
ml
TNTC
TNTC
63
1:100
1:10,000
1:100,000
TNTC
59](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd461c25a-9071-49d5-8112-bf222c78735e%2F68ae1bb8-8a40-415d-9747-b7df93c00869%2Fiho0phq_processed.jpeg&w=3840&q=75)
Transcribed Image Text:5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilution
scheme and include the overall dilution in each tube.
From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After
incubation, you count the plates and get the following results:
What was the number of organisms/ml in the initial culture?
Final dilution
No. of
colonies 0.1
No of
colonies 1
ml
ml
TNTC
TNTC
63
1:100
1:10,000
1:100,000
TNTC
59
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