5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilutie scheme and include the overall dilution in each tube. From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After incubation, you count the plates and get the following results: What was the number of organisms/ml in the initial culture?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilution
scheme and include the overall dilution in each tube.
From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After
incubation, you count the plates and get the following results:
What was the number of organisms/ml in the initial culture?
Final dilution
No. of
colonies 0.1
No of
colonies 1
ml
ml
TNTC
TNTC
63
1:100
1:10,000
1:100,000
TNTC
59
Transcribed Image Text:5. You have performed the following serial dilutions: 1:100, 1:100, 1:10. Diagram this dilution scheme and include the overall dilution in each tube. From each dilution, you then prepare pour plates using either 0.1 ml or 1 ml samples. After incubation, you count the plates and get the following results: What was the number of organisms/ml in the initial culture? Final dilution No. of colonies 0.1 No of colonies 1 ml ml TNTC TNTC 63 1:100 1:10,000 1:100,000 TNTC 59
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