I’m stuck with this question 5. Observe the structure of antibodies below that has four protein subunits. Explain why we onlysee two (not four) bands on the SDS PAGE gel.!1QAZantigenbindingsite2Body*WSconstantregionX#3Evariable..regionD$4ACheavychainRFlightchainB%5antigenbindingsiteVI UTMacBook Pro< 6GYB=>&7HUEN* 008J(9MK)O0

Answered: 5. Observe the structure of antibodies…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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5. Observe the structure of antibodies below that has four protein subunits. Explain why we only
see two (not four) bands on the SDS PAGE gel.
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Expert Solution

Step 1

Introduction

SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a commonly used technique in biochemistry and molecular biology to separate proteins based on their size. It involves placing proteins in a gel matrix and subjecting them to an electric field that causes them to migrate through the gel towards the positively charged electrode.

The gel is made up of polyacrylamide, which acts as a molecular sieve, and a buffer system, which maintains the pH and conductivity necessary for the migration of the proteins.

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