Help! Thank you 5. Not wanting to waste an enzyme, your lab partner mixed 45 µl of a 20 mM enzyme solution with 80 µl of a30 mM enzyme solution. What is the final concentration (in mM) of the resulting mixture?

The two solutions that were mixed are: 1. 45 μL , 20mM of 1st enzyme solution 2. 80 μL , 30mM of 2nd…

Answered: 5. Not wanting to waste an enzyme, your…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 3.3P

See similar textbooks

Similar questions

You have a stock solution containing 1.5 mM of substrate in borate buffer pH 9.5. You transfer 1.25 mL of this stock into a cuvette and then add 250 µl of enzyme solution to initiate the reaction. What will be the final concentration of substrate in the cuvette following addition of the enzyme? 1.5 mM 1.25 mM 0.0075 mM 7.5 mM 1250 mM
Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…
A researcher is preparing a reaction mixture to test the activity of a protein. They combine the required reaction components, which contained in a final 100 ml reaction volume 200 mM NaCl, unknown concentrations of acetic acid and acetate anions and a total [H+] concentration of 790 ricromolar. Can you determine the pH of the solution? Provide the answer to one decimal place. Note: You may need to round the numbers to get the required answer. 100 Strips pH indicator strips non-bleeding pH pH 0-14 EM-Reagents Dip in-read while still moist. immerse in weakly-buffered solutions une there is no further color change (1-10 min) 3 7 5 6
You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?
1
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?
For Table 1: calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.
You have 10 mg/ml ethidium bromide solution. If you add 5 µl of this to 50 ml of agarose gel solution, what will be the final concentration of ethidium bromide in the gel solution in mg/ml?
A researcher is preparing a reaction mixture to test the activity of a protein. They combine at room temperature the required reaction components, which contained in a final 150 ml volume 200 mM NaCl and unknown concentrations of weak acid and conjugate base of a buffer compound. They are using a pH meter to determine the acidity of the solution and observe the following pH value that is displayed by the device. Can you determine the hydrogen ion [H+] concentration of the solution? Provide the value with three decimal places and an appropriate unit in the answer box. Note: You may need to round the numbers to get the required answer. N 3.35
What is the Rf value of each amino acid observed?
Solve the following and explain the complete process.

SEE MORE QUESTIONS

Recommended textbooks for you

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Aquaculture Science

Biology

ISBN:

9781133558347

Author:

Parker

Publisher:

Cengage

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Aquaculture Science

Biology

ISBN:

9781133558347

Author:

Parker

Publisher:

Cengage

SEE MORE TEXTBOOKS

5. Not wanting to waste an enzyme, your lab partner mixed 45 ul of a 20 mM enzyme solution with 80 µl of a 30 mM enzyme solution. What is the final concentration (in mM) of the resulting mixture?