5. Experimental Procedure, Part C.1. Will the addition of NaC,H;O, to a CH;COOH solution cause the pH to increase or decrease? Explain. See equation 16.14.

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5. Experimental Procedure, Part C.1. Will the addition of NaC,H;O, to a CH;COOH solution cause the pH to increase or
decrease? Explain. See equation 16.14.
Transcribed Image Text:5. Experimental Procedure, Part C.1. Will the addition of NaC,H;O, to a CH;COOH solution cause the pH to increase or decrease? Explain. See equation 16.14.
The use of a well plate is recommended. Appropriately labeled 75-mm test tubes are
equally useful for performing the experiments.
C. A Buffer System
7a
7b
1. Preparation of buffered and unbuffered systems. Transfer 10 drops of 0.10 M
CH,COOH to wells Al and A2 of a 24-well plate, (or appropriately labeled 75-mm
test tubes), add 3 drops of universal indicator, and note the color. Compare the
color of the solution with the pH color chart for the universal indicator.☺ Now add
10 drops of 0.10 M NACH,CO, to each well.
Place 20 drops of deionized water into wells B1 and B2 and add 3 drops of uni-
versal indicator.☺
2
Buffer
Buffer
A
HCI
NaOH
2. Effect of strong acid. Add 5-6 drops of 0.10 M HCl to wells Al and B1, estimate
the pH, and record each pH change
3. Effect of strong base. Add 5–6 drops of 0.10 M NaOH to wells A2 and B2, esti-
mate the pH, and record each pH change.
4. Effect of a buffer system. Explain the observed pH change for a buffered system
(as compared with an unbuffered system) when a strong acid or strong base is
added to it.
H20
H20
HCI
NaOH
Transcribed Image Text:The use of a well plate is recommended. Appropriately labeled 75-mm test tubes are equally useful for performing the experiments. C. A Buffer System 7a 7b 1. Preparation of buffered and unbuffered systems. Transfer 10 drops of 0.10 M CH,COOH to wells Al and A2 of a 24-well plate, (or appropriately labeled 75-mm test tubes), add 3 drops of universal indicator, and note the color. Compare the color of the solution with the pH color chart for the universal indicator.☺ Now add 10 drops of 0.10 M NACH,CO, to each well. Place 20 drops of deionized water into wells B1 and B2 and add 3 drops of uni- versal indicator.☺ 2 Buffer Buffer A HCI NaOH 2. Effect of strong acid. Add 5-6 drops of 0.10 M HCl to wells Al and B1, estimate the pH, and record each pH change 3. Effect of strong base. Add 5–6 drops of 0.10 M NaOH to wells A2 and B2, esti- mate the pH, and record each pH change. 4. Effect of a buffer system. Explain the observed pH change for a buffered system (as compared with an unbuffered system) when a strong acid or strong base is added to it. H20 H20 HCI NaOH
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