5. Calculate km and Vmax of the following graphs. Y-axis is the initial rate (uM/min)
5. Calculate km and Vmax of the following graphs. Y-axis is the initial rate (uM/min)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
I need help calculating the km and Vmax of the following graph.
![### Enzyme Kinetics Analysis
#### Task
Calculate \( K_m \) and \( V_{max} \) from the following graphs. The Y-axis represents the initial rate (\( \mu M/min \)).
#### Graph 1: Michaelis-Menten Plot
- **X-Axis**: Substrate concentration \([S]\) in mM
- **Y-Axis**: Initial rate of reaction (\(\mu M/min\))
- **Description**: The graph depicts a hyperbolic curve typical of enzyme kinetics, showing the initial rate of reaction increasing with substrate concentration. This plateau suggests the approach towards \( V_{max} \).
#### Graph 2: Lineweaver-Burk Plot
- **X-Axis**: \( 1/[S] \) (reciprocal of substrate concentration)
- **Y-Axis**: \( 1/v \) (reciprocal of reaction rate)
- **Description**: The plot is a straight line representing the linear transformation of the Michaelis-Menten equation. The y-intercept indicates \( 1/V_{max} \), while the x-intercept corresponds to \(-1/K_m\).
By using these plots, Km and Vmax can be calculated and interpreted to understand enzyme efficiency and affinity in this reaction.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff2188b11-926f-4a02-9047-46f03f4937df%2F784d16bc-f81b-4dfd-90c6-183c6ee65b0b%2Fjwodium_processed.png&w=3840&q=75)
Transcribed Image Text:### Enzyme Kinetics Analysis
#### Task
Calculate \( K_m \) and \( V_{max} \) from the following graphs. The Y-axis represents the initial rate (\( \mu M/min \)).
#### Graph 1: Michaelis-Menten Plot
- **X-Axis**: Substrate concentration \([S]\) in mM
- **Y-Axis**: Initial rate of reaction (\(\mu M/min\))
- **Description**: The graph depicts a hyperbolic curve typical of enzyme kinetics, showing the initial rate of reaction increasing with substrate concentration. This plateau suggests the approach towards \( V_{max} \).
#### Graph 2: Lineweaver-Burk Plot
- **X-Axis**: \( 1/[S] \) (reciprocal of substrate concentration)
- **Y-Axis**: \( 1/v \) (reciprocal of reaction rate)
- **Description**: The plot is a straight line representing the linear transformation of the Michaelis-Menten equation. The y-intercept indicates \( 1/V_{max} \), while the x-intercept corresponds to \(-1/K_m\).
By using these plots, Km and Vmax can be calculated and interpreted to understand enzyme efficiency and affinity in this reaction.
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