5- Select the correct sequence of steps in a proteomic experiment: Protein extraction from the sample, digestion, MS, separation using 2-DE O Protein digestion, separation using 2-DE. MS, peptide sequencing Protein extraction from the sample, isoelectro focusing, SDS-Page, digestion, MS

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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5- Select the correct sequence
of
steps
a proteomic experiment:
Protein extraction from the sample, digestion, MS, separation using 2-DE
Protein digestion, separation using 2-DE, MS, peptide sequencing
Protein extraction from the sample, isoelectro focusing, SDS-Page, digestion, MS
d)
Protein extraction from the sample, separation using 2-DE, digestion, MS
e)
Protein extraction from the sample, digestion, separation using 2-DE. MS
Transcribed Image Text:5- Select the correct sequence of steps a proteomic experiment: Protein extraction from the sample, digestion, MS, separation using 2-DE Protein digestion, separation using 2-DE, MS, peptide sequencing Protein extraction from the sample, isoelectro focusing, SDS-Page, digestion, MS d) Protein extraction from the sample, separation using 2-DE, digestion, MS e) Protein extraction from the sample, digestion, separation using 2-DE. MS
3- Which of the following is NOT true?
a)
Arachidonic acid can be obtained from membrane phospholipids by phospholipase A2
b)
Eicosanoids can be produced from arachidonic acid
C)
Linoleic acid is an essential fatty acid
Oleic acid is a monounsaturated fatty acid
e)
Stearic acid is a polyunsaturated fatty acid
Transcribed Image Text:3- Which of the following is NOT true? a) Arachidonic acid can be obtained from membrane phospholipids by phospholipase A2 b) Eicosanoids can be produced from arachidonic acid C) Linoleic acid is an essential fatty acid Oleic acid is a monounsaturated fatty acid e) Stearic acid is a polyunsaturated fatty acid
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