STEM Workplace Practices 4. Which step in up/downstream processing consists of purification and buffer exchange?

Upstream and downstream processing are two crucial steps in the creation of biopharmaceuticals,…

Answered: 4. Which step in up/downstream…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter20: Applications Of Oxidation/reduction Titrations

Section: Chapter Questions

Problem 20.6QAP

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STEM Workplace Practices

4. Which step in up/downstream processing consists of purification and buffer exchange?

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A 0.300-g feed sample is analyzed for its protein content by the modified Kjeldahl method. If 25.0 mL of 0.100 M HCl is required for titration, what is the percent protein content of the sample?
✓ ?? 14. Two groups of students were asked to check the concentration of ammonia in a particular brand of cleaning product because there had been complaints about that brand. The students placed a 25.0mL sample of cleaning product in a volumetric flask and made it up to 250mL using distilled water. They then performed a titration by using 25.0mL of the diluted ammonia solution in a conical flask and 0.10 moll-¹ standardised hydrochloric acid in a burette. One group used bromocresol green (pH range 4.0-5.6) as the indicator and obtained the following results: Titration Volume of acid 1st 23.2 added (mL) 2nd 22.9 3rd 22.8 4th 22.9 a) Calculate the concentration of the diluted sample. b) Calculate the concentration of ammonia in 1L of the original sample.
What is the importance of non-aqueous titrations in pharmaceutical analysis?
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Acid-Base titration: Standardization of 0.1 M NAOH Trial 1 Mass KHP used, g 0.297 g Final reading Volume NaOH, mL 15.59 mL Initial reading Volume NaOH, mL 0.23 mL Volume NaOH consumed, mL 15.36 mL Moles KHP used Moles NaOH used Molarity of NaOH solution
1. Molarity of the NaOH solution 0.238 mol/L Trial 1 Trial 2 Trial 2. Volume of H;PO4 added to flask 22.0 mL 22.0 mL 22.0 mL 3. Initial NaOH volume _0.25_ mL _0.75_ mL 0.55 mL 4. Final NaOH volume 17.60_ mL 18.20_ mL _17.90_ mL 5. NAOH volume used for titration to reach green end point mL mL mL 6. NaOH volume used for titration 7. Moles of NaOH used for titration mol mel mel 8. Moles of H;PO4 that reacted mol mel mol 9. Volume of H;PO4 added to flask L 10. Molarity of H3PO4 mol/L mol/L mol/L
The purpose of adding an excess of the precipitating agent is: 1- Ensure that the sedimentation is complete 2- Get big crystals 3- Purification of the precipitate 4-Prevent pollution
By reference to the reacting ratio of HCl and NaOH, deduce the number of moles of NaOH required to reach equivalence.
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Table 12.9 (data) Buffer Initial pH pH after adding HCI pH after adding NaOH 6 8 6 4 Solution A 5-6 8 Solution B8-9 10 Solution C7 9-10 Solution D 5 Boiled distilled water 5 9-10 4 9 Table 12.10 (report) Solutions able to buffer well against acid Solutions able to buffer well against base Solutions able to buffer well against acid or base Solutions with little or no buffering ability against acid or base
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