4. Draw the titration curve of Glu (pKa for α-NH, is 9.3, pKa for a-COOH is 1.8, and pKR side chain is 4.0) 14 Hd HO 6 5 4 43210987654325 13 12 11 10 9 8 0.0 0.5 1.0 1.5 2.0 2.5 3.0 moles of OH- added/mole Glu 5. Draw dipeptide Arg-Thr at pH 6.0. Circle the six atoms that are restricted in one plane. Calculate its approximate p/ assuming pKa for NH3+ is 10.0, pKa for COOH is 3.0, PKR for Arg is 12.0 6. In what order would the following molecules be eluted from a gel exclusion column at pH 8.0? List the order from first to last. a. Horse Cytochrome C (MW: 12.4 kD) b. Horse Myoglobin (MW: 17 kD) C. Rabbit Hemoglobin (MW: 68 kD) d. Blue Dextran (MW: 2000 kD)
4. Draw the titration curve of Glu (pKa for α-NH, is 9.3, pKa for a-COOH is 1.8, and pKR side chain is 4.0) 14 Hd HO 6 5 4 43210987654325 13 12 11 10 9 8 0.0 0.5 1.0 1.5 2.0 2.5 3.0 moles of OH- added/mole Glu 5. Draw dipeptide Arg-Thr at pH 6.0. Circle the six atoms that are restricted in one plane. Calculate its approximate p/ assuming pKa for NH3+ is 10.0, pKa for COOH is 3.0, PKR for Arg is 12.0 6. In what order would the following molecules be eluted from a gel exclusion column at pH 8.0? List the order from first to last. a. Horse Cytochrome C (MW: 12.4 kD) b. Horse Myoglobin (MW: 17 kD) C. Rabbit Hemoglobin (MW: 68 kD) d. Blue Dextran (MW: 2000 kD)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:4. Draw the titration curve of Glu (pKa for α-NH, is 9.3, pKa for a-COOH is 1.8, and pKR side chain
is 4.0)
14
Hd
HO
6
5
4
43210987654325
13
12
11
10
9
8
0.0
0.5
1.0
1.5
2.0
2.5
3.0
moles of OH- added/mole Glu
5. Draw dipeptide Arg-Thr at pH 6.0. Circle the six atoms that are restricted in one plane.
Calculate its approximate p/ assuming pKa for NH3+ is 10.0, pKa for COOH is 3.0, PKR for Arg is
12.0
6. In what order would the following molecules be eluted from a gel exclusion column at pH 8.0? List
the order from first to last.
a.
Horse Cytochrome C (MW: 12.4 kD)
b.
Horse Myoglobin (MW: 17 kD)
C.
Rabbit Hemoglobin (MW: 68 kD)
d.
Blue Dextran (MW: 2000 kD)
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