Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition....Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys....to the double mutant form....Lys-Val-His- His-Leu-Met-Ala-Lys.... (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant (b) Which base was added? (c) Which base was deleted?

4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition....Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys....to the double mutant form....Lys-Val-His- His-Leu-Met-Ala-Lys.... (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant (b) Which base was added? (c) Which base was deleted?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Just Question 4
Certainly! Here's the transcription for an educational website format: --- **Topic: Genetic Code and Mutations** 2. **Question:** How many different mRNAs could specify the amino acid sequence Met-Phe-Ser-Pro? List them. 3. **Question:** If the coding region of a gene is estimated to consist of 450 bp, how many amino acids would the corresponding polypeptide chain contain? Estimate the molecular weight of the polypeptide chain (assume the average molecular weight of an amino acid is 110 Daltons). 4. **Mutation Study:** A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition,…Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys… to the double mutant form…Lys-Val-His-His-Leu-Met-Ala-Lys…. (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for the original (wild-type) protein and the double mutant. (b) Which base was added? (c) Which base was deleted? 5. **Eukaryotic Cell Mutation:** A eukaryotic cell produces a normal protein with the following amino acid sequence: Met-Val-His-Lys-Arg-Thr-Leu-Val. After irradiation, a transformed (mutated) eukaryotic cell is found to produce a mutant protein from the same coding site on DNA: Met-Val-His-Lys-Glu-Pro. Describe the mutation that occurred. As always, justify your answer. 6. **Mutagen Analysis:** You are working with a newly discovered mutagen, and you wish to determine the base change that it introduced into the DNA. Thus far you have determined that the mutagen chemically alters a single base in such a way that its base-pairing properties are altered permanently. In order to determine the mutation type, you sequence the mRNA and examine amino acid changes that place after mutagenesis. A typical example is presented below: - **Wild-type:** Gln-His-Ile-Glu-Ser Gln-His-Met-Glu-Ser Gln-Ile-Met-Glu-Ser - **Mutant:** Arg
Transcribed Image Text:Certainly! Here's the transcription for an educational website format: --- **Topic: Genetic Code and Mutations** 2. **Question:** How many different mRNAs could specify the amino acid sequence Met-Phe-Ser-Pro? List them. 3. **Question:** If the coding region of a gene is estimated to consist of 450 bp, how many amino acids would the corresponding polypeptide chain contain? Estimate the molecular weight of the polypeptide chain (assume the average molecular weight of an amino acid is 110 Daltons). 4. **Mutation Study:** A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition,…Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys… to the double mutant form…Lys-Val-His-His-Leu-Met-Ala-Lys…. (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for the original (wild-type) protein and the double mutant. (b) Which base was added? (c) Which base was deleted? 5. **Eukaryotic Cell Mutation:** A eukaryotic cell produces a normal protein with the following amino acid sequence: Met-Val-His-Lys-Arg-Thr-Leu-Val. After irradiation, a transformed (mutated) eukaryotic cell is found to produce a mutant protein from the same coding site on DNA: Met-Val-His-Lys-Glu-Pro. Describe the mutation that occurred. As always, justify your answer. 6. **Mutagen Analysis:** You are working with a newly discovered mutagen, and you wish to determine the base change that it introduced into the DNA. Thus far you have determined that the mutagen chemically alters a single base in such a way that its base-pairing properties are altered permanently. In order to determine the mutation type, you sequence the mRNA and examine amino acid changes that place after mutagenesis. A typical example is presented below: - **Wild-type:** Gln-His-Ile-Glu-Ser Gln-His-Met-Glu-Ser Gln-Ile-Met-Glu-Ser - **Mutant:** Arg
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS