3.3. The enzyme, fumarase, has the following kinetic constants: S+E = ES P+E k- where k = 10° M-' s-' k_1 = 4.4 × 10ª s¬! k2 = 10° s-' a. What is the value of the Michaelis constant for this enzyme? b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at a substrate concentration of 10-³ M?
3.3. The enzyme, fumarase, has the following kinetic constants: S+E = ES P+E k- where k = 10° M-' s-' k_1 = 4.4 × 10ª s¬! k2 = 10° s-' a. What is the value of the Michaelis constant for this enzyme? b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at a substrate concentration of 10-³ M?
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![3.3. The enzyme, fumarase, has the following kinetic constants:
k,
S+E = ES-
→P+E
k-,
where k, = 10° M-' s-'
k_ = 4.4 x 104 s-1
k2 = 10° s-'
a. What is the value of the Michaelis constant for this enzyme?
b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at
a substrate concentration of 10-3 M?
[Courtesy of D. J. Kirwan from "Collected Coursework Problems in Biochemical Engi-
neering" compiled by H. W. Blanch for 1977 Am. Soc. Eng. Educ. Summer School.]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F53062fd9-1948-412d-8d49-8f7d604d0d34%2F110a84d9-9ccd-4916-b7e0-a9025e6d8703%2Fc69h6fk_processed.png&w=3840&q=75)
Transcribed Image Text:3.3. The enzyme, fumarase, has the following kinetic constants:
k,
S+E = ES-
→P+E
k-,
where k, = 10° M-' s-'
k_ = 4.4 x 104 s-1
k2 = 10° s-'
a. What is the value of the Michaelis constant for this enzyme?
b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at
a substrate concentration of 10-3 M?
[Courtesy of D. J. Kirwan from "Collected Coursework Problems in Biochemical Engi-
neering" compiled by H. W. Blanch for 1977 Am. Soc. Eng. Educ. Summer School.]
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