3. You are given a mixture of four proteins, whose properties are listed in the table below. Propose a process to purify each protein so that you end up with four solutions of pure protein. What resin would you use to bind the protein(s)? What changes to the buffer would you make to desorb the protein(s)? Contains an N-terminal His6-tag. Two 50 kDa subunits contain a non-heme Fe2+ in the active site. Protein Size (kDa) pl Specific Properties A 100 6.0 B 40 7.7 C 240 8.3 Ꭰ 225 5.5 Contains FAD redox center and an NADH binding domain. Composed of six 40-kDa subunits, each of which contains a [2Fe-2S] cluster. Composed of three subunits: 100 kDa structural subunit, 75 kDa subunit with a molybdopterin center, and 50 kDa subunit with FAD and an NADH binding domain.
3. You are given a mixture of four proteins, whose properties are listed in the table below. Propose a process to purify each protein so that you end up with four solutions of pure protein. What resin would you use to bind the protein(s)? What changes to the buffer would you make to desorb the protein(s)? Contains an N-terminal His6-tag. Two 50 kDa subunits contain a non-heme Fe2+ in the active site. Protein Size (kDa) pl Specific Properties A 100 6.0 B 40 7.7 C 240 8.3 Ꭰ 225 5.5 Contains FAD redox center and an NADH binding domain. Composed of six 40-kDa subunits, each of which contains a [2Fe-2S] cluster. Composed of three subunits: 100 kDa structural subunit, 75 kDa subunit with a molybdopterin center, and 50 kDa subunit with FAD and an NADH binding domain.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![3. You are given a mixture of four proteins, whose properties are listed in the table below.
Propose a process to purify each protein so that you end up with four solutions of pure
protein. What resin would you use to bind the protein(s)? What changes to the buffer would
you make to desorb the protein(s)?
Contains an N-terminal His6-tag. Two 50 kDa subunits
contain a non-heme Fe2+ in the active site.
Protein Size (kDa) pl
Specific Properties
A
100
6.0
B
40
7.7
C
240
8.3
Ꭰ
225
5.5
Contains FAD redox center and an NADH binding
domain.
Composed of six 40-kDa subunits, each of which
contains a [2Fe-2S] cluster.
Composed of three subunits: 100 kDa structural subunit,
75 kDa subunit with a molybdopterin center, and 50 kDa
subunit with FAD and an NADH binding domain.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F76a241b1-3148-428a-9d7b-4a1276447a9a%2F27a8de1c-9368-423b-b1cb-1aa042385bf6%2F1k8uqus_processed.jpeg&w=3840&q=75)
Transcribed Image Text:3. You are given a mixture of four proteins, whose properties are listed in the table below.
Propose a process to purify each protein so that you end up with four solutions of pure
protein. What resin would you use to bind the protein(s)? What changes to the buffer would
you make to desorb the protein(s)?
Contains an N-terminal His6-tag. Two 50 kDa subunits
contain a non-heme Fe2+ in the active site.
Protein Size (kDa) pl
Specific Properties
A
100
6.0
B
40
7.7
C
240
8.3
Ꭰ
225
5.5
Contains FAD redox center and an NADH binding
domain.
Composed of six 40-kDa subunits, each of which
contains a [2Fe-2S] cluster.
Composed of three subunits: 100 kDa structural subunit,
75 kDa subunit with a molybdopterin center, and 50 kDa
subunit with FAD and an NADH binding domain.
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