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3. What is the optimum pH of the enzyme in the saliva?

3. What is the optimum pH of the enzyme in the saliva?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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3. What is the optimum pH of the enzyme in the saliva?
Transcribed Image Text:3. What is the optimum pH of the enzyme in the saliva?
Reagents: Saliva, 30 ml 1% starch, 1 ml 0.05N HCI, 1 ml 0.05N NaOH, 2 ml iodine in KI, 3ml benedict's reagent, 1 ml distilled water, 3 ml 0.01M NaCI Materials: 6 Test tubes (20 ml), test tube brush, test tube holder, test tube rack, 10 ml graduated cylinder, thermometer, alcohol lamp, tripod, wire gauze, beaker, spot plate, 4 dropper, stirring rod Procedure: 1. Label 3 test tubes 1, 2 and 3. Place the substances in each test tube as indicated below: Tube 1% starch 0.1M NaCl Acid/base/water Saliva 1 10 ml 1 ml 1ml 0.05N HCI 2 ml 1 ml distilled H2O 10 ml 1ml 2 ml 1 ml 0.05N 3 10 ml 1 ml 2 ml NaOH 2. Mix well by shaking each tube and place in a water bath with temperature maintained at 40°C for 30 minutes. 3. Remove all tubes from the water bath and stir. 4. Perform iodine test by taking 1 drop from each tube and place in different holes of a spot plate. Add 1 drop iodine and mix. Note color. 5. Perform benedicts test. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 6. If the result in iodine test is positive (violet to blue color) and the benedicts test is negative return the tubes in the water bath and wait for another 15 minutes. 7. Repeat procedure 4until the blue color of starch with iodine fail to appear. Note the time of the disappearance of the blue color in iodine test. 8. Then perform Benedict's test by placing 1 ml of benedicts reagent in a test tube and add 3 drops of the sample (that is already negative to iodine). Heat in a boiling water bath for 2 mins. Note color of precipitate formed. 9. If no brick red precipitate is formed, return the tube in the water bath (40°C) for 15 minutes. 10. Repeat procedures 8-9 until a brick red precipitate is obtained. Note time of the appearance of the precipitate.
Transcribed Image Text:Reagents: Saliva, 30 ml 1% starch, 1 ml 0.05N HCI, 1 ml 0.05N NaOH, 2 ml iodine in KI, 3ml benedict's reagent, 1 ml distilled water, 3 ml 0.01M NaCI Materials: 6 Test tubes (20 ml), test tube brush, test tube holder, test tube rack, 10 ml graduated cylinder, thermometer, alcohol lamp, tripod, wire gauze, beaker, spot plate, 4 dropper, stirring rod Procedure: 1. Label 3 test tubes 1, 2 and 3. Place the substances in each test tube as indicated below: Tube 1% starch 0.1M NaCl Acid/base/water Saliva 1 10 ml 1 ml 1ml 0.05N HCI 2 ml 1 ml distilled H2O 10 ml 1ml 2 ml 1 ml 0.05N 3 10 ml 1 ml 2 ml NaOH 2. Mix well by shaking each tube and place in a water bath with temperature maintained at 40°C for 30 minutes. 3. Remove all tubes from the water bath and stir. 4. Perform iodine test by taking 1 drop from each tube and place in different holes of a spot plate. Add 1 drop iodine and mix. Note color. 5. Perform benedicts test. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 6. If the result in iodine test is positive (violet to blue color) and the benedicts test is negative return the tubes in the water bath and wait for another 15 minutes. 7. Repeat procedure 4until the blue color of starch with iodine fail to appear. Note the time of the disappearance of the blue color in iodine test. 8. Then perform Benedict's test by placing 1 ml of benedicts reagent in a test tube and add 3 drops of the sample (that is already negative to iodine). Heat in a boiling water bath for 2 mins. Note color of precipitate formed. 9. If no brick red precipitate is formed, return the tube in the water bath (40°C) for 15 minutes. 10. Repeat procedures 8-9 until a brick red precipitate is obtained. Note time of the appearance of the precipitate.
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