Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
3. What is the importance of the iodine and benedict’s tests in this activity?

3. What is the importance of the iodine and benedict’s tests in this activity?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
100%

Number 3 please

EFFECT OF ENΖΥΜΕ CONCENTRATIOΝ ON ΕNZΥΜΕ ACTIVITY Introduction: Enzymes are highly specialized class of proteins. They catalyze thousands of chemical reactions with remarkable property of speeding up chemical reactions. They combine with the reactants to produce a transition state having less energy than the transition state of uncatalyzed reaction. When reaction products are formed the free catalyst is regenerated. The rate of enzyme catalyzed reaction is determined by the concentration of the enzyme used. The more concentrated the enzyme used, the faster is the reaction. Objectives: At the end of the activity, the student can: 1. Determine the effect of different concentration of enzyme on reaction rate 2. Compute for the concentration of enzyme present in each tube. 3. Determine in which concentration reaction rate is fastest and slowest. Reagents: Saliva, 6 ml 1% starch solution, 6 drops Iz in KI, 6 ml benedict's reagent. Materials: 12 Test tube (20 ml), 10 ml graduated cylinder, test tube rack, brush, 250 ml beaker, tripod, wire gauze, thermometer, alcohol lamp Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add 1ml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
Transcribed Image Text:EFFECT OF ENΖΥΜΕ CONCENTRATIOΝ ON ΕNZΥΜΕ ACTIVITY Introduction: Enzymes are highly specialized class of proteins. They catalyze thousands of chemical reactions with remarkable property of speeding up chemical reactions. They combine with the reactants to produce a transition state having less energy than the transition state of uncatalyzed reaction. When reaction products are formed the free catalyst is regenerated. The rate of enzyme catalyzed reaction is determined by the concentration of the enzyme used. The more concentrated the enzyme used, the faster is the reaction. Objectives: At the end of the activity, the student can: 1. Determine the effect of different concentration of enzyme on reaction rate 2. Compute for the concentration of enzyme present in each tube. 3. Determine in which concentration reaction rate is fastest and slowest. Reagents: Saliva, 6 ml 1% starch solution, 6 drops Iz in KI, 6 ml benedict's reagent. Materials: 12 Test tube (20 ml), 10 ml graduated cylinder, test tube rack, brush, 250 ml beaker, tripod, wire gauze, thermometer, alcohol lamp Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add 1ml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
3. What is the importance of the iodine and benedict’s tests in this activity? Iodine test Benedict's test
Transcribed Image Text:3. What is the importance of the iodine and benedict’s tests in this activity? Iodine test Benedict's test
Expert Solution
steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS