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3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol) contained in a head about 100 nanometers (nm) long. (a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650 g/mol) and compare it with the length of the JS98 head. (b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What is the exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows the exact format as described in the Biology Style Manual).

3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol) contained in a head about 100 nanometers (nm) long. (a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650 g/mol) and compare it with the length of the JS98 head. (b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What is the exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows the exact format as described in the Biology Style Manual).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linking number (Lk) of 374? What is the superhelical density (o) of the same DNA when Lk = 412? In each case, is the DNA negatively or positively supercoiled? S 2. The complete genome of the simplest bacterium known, Mycoplasma genitalium, is a circular DNA molecule with 580,070 bp. Calculate the molecular weight (assume the molecular weight of a nucleotide pair is 650 g/mol) and contour length (when relaxed) of this molecule. What is Lko for this Mycoplasma chromosome? @ 2 W 3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol) contained in a head about 100 nanometers (nm) long. (a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650 g/mol) and compare it with the length of the JS98 head. (b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What is the exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows the exact format as described in the Biology Style Manual). 4. A closed-circular DNA molecule in its relaxed form has an Lk of 500. Approximately how many base pairs are in this DNA? How does the Lk change (increases, decreases, does not change, becomes undefined) in each of the following situations? (a) A protein complex binds, wrapping the DNA round it to form a solenoidal supercoil. (b) One DNA strand is broken. # 3 E (c) DNA gyrase and ATP are added to the DNA in solution. (d) The double helix is denatured by heat. D 4 C $ 4 R F V All s do in % 5 T G 101 ^ < 6 B FG Y H * F7 & 7 U N Presen J ★ 8 Home 1 M ( 9 K End F10 V. ) PgUp L PgOn Alt
Transcribed Image Text:1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a linking number (Lk) of 374? What is the superhelical density (o) of the same DNA when Lk = 412? In each case, is the DNA negatively or positively supercoiled? S 2. The complete genome of the simplest bacterium known, Mycoplasma genitalium, is a circular DNA molecule with 580,070 bp. Calculate the molecular weight (assume the molecular weight of a nucleotide pair is 650 g/mol) and contour length (when relaxed) of this molecule. What is Lko for this Mycoplasma chromosome? @ 2 W 3. The T4-like bacteriophage JS98 has a DNA of molecular weight 1.11 x 108 grams/mole (g/mol) contained in a head about 100 nanometers (nm) long. (a) Calculate the length of the DNA molecule (assume the molecular weight of a nucleotide pair is 650 g/mol) and compare it with the length of the JS98 head. (b) Consult the online database Genome (https://www.ncbi.nlm.nih.gov/data-hub/genome/). What is the exact number of base pairs in the JS98 genome? Provide a citation (make sure it follows the exact format as described in the Biology Style Manual). 4. A closed-circular DNA molecule in its relaxed form has an Lk of 500. Approximately how many base pairs are in this DNA? How does the Lk change (increases, decreases, does not change, becomes undefined) in each of the following situations? (a) A protein complex binds, wrapping the DNA round it to form a solenoidal supercoil. (b) One DNA strand is broken. # 3 E (c) DNA gyrase and ATP are added to the DNA in solution. (d) The double helix is denatured by heat. D 4 C $ 4 R F V All s do in % 5 T G 101 ^ < 6 B FG Y H * F7 & 7 U N Presen J ★ 8 Home 1 M ( 9 K End F10 V. ) PgUp L PgOn Alt
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The size of the Genome of an organism defines the total number of base pairs contained within its chromosomes. It can be expressed in base pairs, kilobases; megabases or picograms.

 

 

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