3. Based on his findings at the farm, the USDA inspector was also conçerned that the milk could be contaminated with E. coli 0157 H7 (a pathogenic E. coli strain). Therefore the lab took a sample of the milk and used it to inoculate the medium shown below. (a) What is the name of this medium? (Provide both the acronym and the name spelled out). (b) What does this result tell you about the characteristics of the bacteria on this plate? (Make sure you describe all of the characteristics!) (c) Is the USDA inspector's concern about E. coli O157:H7 justified? Explain! D Focus

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

Please answer only question #3 with the plate

3. Based on his findings at the farm, the USDA inspector was also concerned that the milk could
be contaminated with E. coli 0157 H7 (a pathogenic E. coli strain). Therefore the lab took a
sample of the milk and used it to inoculate the medium shown below.
AM SRmib
(a) What is the name of this medium? (Provide both the acronym and the name spelled out).
(b) What does this result tell you about the characteristics of the bacteria on this plate? (Make
sure you describe all of the characteristics!)
+ 100%
(c) Is the USDA inspector's concern about E. coli Ol57:H7 justified? Explain!
DFocus
04:01 AM
11/19/2020
W
a
0 門
Transcribed Image Text:3. Based on his findings at the farm, the USDA inspector was also concerned that the milk could be contaminated with E. coli 0157 H7 (a pathogenic E. coli strain). Therefore the lab took a sample of the milk and used it to inoculate the medium shown below. AM SRmib (a) What is the name of this medium? (Provide both the acronym and the name spelled out). (b) What does this result tell you about the characteristics of the bacteria on this plate? (Make sure you describe all of the characteristics!) + 100% (c) Is the USDA inspector's concern about E. coli Ol57:H7 justified? Explain! DFocus 04:01 AM 11/19/2020 W a 0 門
Comments
AaBbCcC AaB
Find
ormal
T No Spac. Heading 1 Heading 2
Replace
Title
Paragraph
A Select
Dictate
Editor
Styles
Editing
Voice
Editor
A USDA inspector has been tasked with investigating a dairy whose pasteurization
processes are suspect. After inspecting the farm and the milking sheds, the inspector took
several samples of pasteurized milk and sent them to the lab for analysis.
At the lab serial dilutions were done to determine the CFUS/ml in the milk sample. The
standards for safe milk in NY State require that bacterial counts for pasteurized milk should not
exceed 20,000 CFUS/ml. The total coliform amount should not exceed 10 CFUS/ml.
1. Given the amount of bacteria that could be present in the pasteurized milk, explain why it
would be necessary to use serial dilutions to accurately determine the CFUS/ml.
2. The procedure used by the lab for the serial dilutions is shown below. 0.2 ml from each of the
indicated dilutions was used to inoculate plates. The CFUS/plate are indicated. Note: pay verv
close attention to how the dilutions were done!
(a) What is the dilution factor for each tube (1-5)?
(b) Determine the number of CFUS/ml in the pasteurized milk from the dairy. Show your work!
(c) Is the dairy in compliance with NY State regulations regarding milk quality? Explain your
answer!
1 ml
1 ml
1 ml
Transcribed Image Text:Comments AaBbCcC AaB Find ormal T No Spac. Heading 1 Heading 2 Replace Title Paragraph A Select Dictate Editor Styles Editing Voice Editor A USDA inspector has been tasked with investigating a dairy whose pasteurization processes are suspect. After inspecting the farm and the milking sheds, the inspector took several samples of pasteurized milk and sent them to the lab for analysis. At the lab serial dilutions were done to determine the CFUS/ml in the milk sample. The standards for safe milk in NY State require that bacterial counts for pasteurized milk should not exceed 20,000 CFUS/ml. The total coliform amount should not exceed 10 CFUS/ml. 1. Given the amount of bacteria that could be present in the pasteurized milk, explain why it would be necessary to use serial dilutions to accurately determine the CFUS/ml. 2. The procedure used by the lab for the serial dilutions is shown below. 0.2 ml from each of the indicated dilutions was used to inoculate plates. The CFUS/plate are indicated. Note: pay verv close attention to how the dilutions were done! (a) What is the dilution factor for each tube (1-5)? (b) Determine the number of CFUS/ml in the pasteurized milk from the dairy. Show your work! (c) Is the dairy in compliance with NY State regulations regarding milk quality? Explain your answer! 1 ml 1 ml 1 ml
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Types of communication
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education