3. Based on his findings at the farm, the USDA inspector was also conçerned that the milk could be contaminated with E. coli 0157 H7 (a pathogenic E. coli strain). Therefore the lab took a sample of the milk and used it to inoculate the medium shown below. (a) What is the name of this medium? (Provide both the acronym and the name spelled out). (b) What does this result tell you about the characteristics of the bacteria on this plate? (Make sure you describe all of the characteristics!) (c) Is the USDA inspector's concern about E. coli O157:H7 justified? Explain! D Focus

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Please answer only question #3 with the plate

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Transcribed Image Text:I'm sorry, I can't transcribe the text from the image.
**Investigation of Dairy Pasteurization Processes**

A USDA inspector is tasked with investigating a dairy whose pasteurization processes are suspect. After inspecting the farm and the milking sheds, the inspector collected several samples of pasteurized milk and sent them to the lab for analysis.

**Standards and Requirements:**
- New York State standards for safe milk require that the bacterial counts for CFUs/ml should not exceed 20,000 CFUs/ml.
- The total coliform amount should not exceed 10 CFUs/ml.

**Tasks:**

1. **Research Question:**
   - **Given the amount of bacteria that could be present in the pasteurized milk, explain why it would be necessary to use serial dilutions to accurately determine the CFUs/ml.**

2. **Laboratory Procedure:**
   - The procedure used for serial dilutions is given below. 0.2 ml from each of the indicated dilutions was used to inoculate plates. The CFUs/plate are noted.
   - **Note:** Pay very close attention to how the dilutions were done.

   **Questions:**
   - (a) What is the dilution factor for each tube (1-5)?
   - (b) Determine the number of CFUs/ml in the pasteurized milk from the dairy. Show your work!
   - (c) Is the dairy in compliance with New York State regulations regarding milk quality? Explain your answer!

**Graph/Diagram:**
- The image contains a series of test tubes labeled 1 ml and a formula represented by multiple arrows leading into subsequent tubes, showing serial dilution. Each dilution step involves mixing with 9 ml of a diluent.

This task involves calculating dilution factors and bacterial counts to ensure that milk safety standards are being met.
Transcribed Image Text:**Investigation of Dairy Pasteurization Processes** A USDA inspector is tasked with investigating a dairy whose pasteurization processes are suspect. After inspecting the farm and the milking sheds, the inspector collected several samples of pasteurized milk and sent them to the lab for analysis. **Standards and Requirements:** - New York State standards for safe milk require that the bacterial counts for CFUs/ml should not exceed 20,000 CFUs/ml. - The total coliform amount should not exceed 10 CFUs/ml. **Tasks:** 1. **Research Question:** - **Given the amount of bacteria that could be present in the pasteurized milk, explain why it would be necessary to use serial dilutions to accurately determine the CFUs/ml.** 2. **Laboratory Procedure:** - The procedure used for serial dilutions is given below. 0.2 ml from each of the indicated dilutions was used to inoculate plates. The CFUs/plate are noted. - **Note:** Pay very close attention to how the dilutions were done. **Questions:** - (a) What is the dilution factor for each tube (1-5)? - (b) Determine the number of CFUs/ml in the pasteurized milk from the dairy. Show your work! - (c) Is the dairy in compliance with New York State regulations regarding milk quality? Explain your answer! **Graph/Diagram:** - The image contains a series of test tubes labeled 1 ml and a formula represented by multiple arrows leading into subsequent tubes, showing serial dilution. Each dilution step involves mixing with 9 ml of a diluent. This task involves calculating dilution factors and bacterial counts to ensure that milk safety standards are being met.
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