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3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution.

3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution. 4. If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your initial techniques for isolating these proteins?
Transcribed Image Text:3. An enzyme is found to have an active site cysteine residue that plays a critical role in catalysis. At what pH does this cysteine operate efficiently in nucleophilic catalysis? Describe how proteins alter the pK of this side chain from its normal value in solution. 4. If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your initial techniques for isolating these proteins?
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