2. Write a balanced chemical equation for the reac- tion of bromine and triolein, a triglyceride of oleic acid, CH3(CH2),CH=CH(CH2),COOH.

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505
REAC 447: Qualitative Testing for Lipids
Post-Laboratory Questions
(Use the spaces provided for the answers and additional paper if necessary.)
Lecithin
A student used the procedure described in this
experiment to try to determine the identity of an un-
known. The lipid formed an emulsion when mixed with
oil and water, reacted with aqueous sodium hydroxide
to form soap, did not react with bromine, and gave a
positive result for the ammonium molybdate test. De-
scribe a lipid structure consistent with these results.
1. Explain what happened when you added lecithin
to the mixture of oll and water. Identify the structural
features of lecithin molecules responsible for this
result.
3.
Hbecame a cloudvorange
Solution blcitlowers,
Cholesterol. Itis not
a positive result
Cholestero!
2. Write a balanced chemical equation for the reac-
tion of bromine and triolein, a triglyceride of oleic acid,
CH3(CH2),CH=CH(CH2),COOH.
4. The structure of N-palmitoyl-D-sphingomyelin, a
sphingolipid, is shown at the bottom of this page. De-
scribe the expected results of the following tests or re-
Cs7Hio4 Ou:38r2-7057HiouD6 Bru agents on thie sphingolipid.
(1) grease-spot test
It remains the Same as room
temp 4develop sgrease-spot
(2) 5% bromine in dichloromethane
Color of th solution
decolori zes
(3) ammonium molybdate
Positive result for sphingolipid
bublole layeracolored
OH
CH-CH=CH(CH2)12CH3
CH3(CH2)14C-NH -CH
CH2-0-P-0-CH,CH2-Ň(CH3)3
Haley Doyle
416122
name
section
date
Transcribed Image Text:505 REAC 447: Qualitative Testing for Lipids Post-Laboratory Questions (Use the spaces provided for the answers and additional paper if necessary.) Lecithin A student used the procedure described in this experiment to try to determine the identity of an un- known. The lipid formed an emulsion when mixed with oil and water, reacted with aqueous sodium hydroxide to form soap, did not react with bromine, and gave a positive result for the ammonium molybdate test. De- scribe a lipid structure consistent with these results. 1. Explain what happened when you added lecithin to the mixture of oll and water. Identify the structural features of lecithin molecules responsible for this result. 3. Hbecame a cloudvorange Solution blcitlowers, Cholesterol. Itis not a positive result Cholestero! 2. Write a balanced chemical equation for the reac- tion of bromine and triolein, a triglyceride of oleic acid, CH3(CH2),CH=CH(CH2),COOH. 4. The structure of N-palmitoyl-D-sphingomyelin, a sphingolipid, is shown at the bottom of this page. De- scribe the expected results of the following tests or re- Cs7Hio4 Ou:38r2-7057HiouD6 Bru agents on thie sphingolipid. (1) grease-spot test It remains the Same as room temp 4develop sgrease-spot (2) 5% bromine in dichloromethane Color of th solution decolori zes (3) ammonium molybdate Positive result for sphingolipid bublole layeracolored OH CH-CH=CH(CH2)12CH3 CH3(CH2)14C-NH -CH CH2-0-P-0-CH,CH2-Ň(CH3)3 Haley Doyle 416122 name section date
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