2. What potential problem can a student have when an R₁ = 0 is obtained for an unknown sample on a silica TLC plate developed with a hexane mixture. How can this problem be avoided involving another TLC experiment?

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**Question 2: TLC Plate Analysis**

**Problem:**  
What potential problem can a student encounter when an \( R_f = 0 \) is obtained for an unknown sample on a silica TLC plate developed with a hexane mixture?

**Solution:**  
The issue here is that the compound did not move from the origin, indicating it is too polar to be eluted by the non-polar hexane solvent. To resolve this, consider using a more polar solvent or solvent mixture to increase compound movement during the TLC experiment.
Transcribed Image Text:**Question 2: TLC Plate Analysis** **Problem:** What potential problem can a student encounter when an \( R_f = 0 \) is obtained for an unknown sample on a silica TLC plate developed with a hexane mixture? **Solution:** The issue here is that the compound did not move from the origin, indicating it is too polar to be eluted by the non-polar hexane solvent. To resolve this, consider using a more polar solvent or solvent mixture to increase compound movement during the TLC experiment.
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