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2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/ml Volume of standard solution to be prepared needed V, (uL) Diluted concentration Volume of Volume of dilution needed solvent needed C; (mg/mL) V2 (uL) V-50-V (uL) 2.0 50 50 2.0 0.05 50 DI D2 D3 2.0 0.1 50 50 2.0 0.2 0.3 0.4 D4 2.0 2.0 50 50 D5 D6 2.0 0.5 50

2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/ml Volume of standard solution to be prepared needed V, (uL) Diluted concentration Volume of Volume of dilution needed solvent needed C; (mg/mL) V2 (uL) V-50-V (uL) 2.0 50 50 2.0 0.05 50 DI D2 D3 2.0 0.1 50 50 2.0 0.2 0.3 0.4 D4 2.0 2.0 50 50 D5 D6 2.0 0.5 50

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/mL Volume of Diluted concentration to be prepared C; (mg/mL) Volume of Volume of standard solution dilution needed solvent needed needed V2 (uL) V=50-V, (uL) V, (uL) 2.0 50 50 2.0 0.05 0.1 0.2 DI 50 D2 2.0 50 2.0 D3 D4 50 2.0 0.3 50 D5 2.0 0.4 50 D6 2.0 0.5 50
Transcribed Image Text:2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/mL Volume of Diluted concentration to be prepared C; (mg/mL) Volume of Volume of standard solution dilution needed solvent needed needed V2 (uL) V=50-V, (uL) V, (uL) 2.0 50 50 2.0 0.05 0.1 0.2 DI 50 D2 2.0 50 2.0 D3 D4 50 2.0 0.3 50 D5 2.0 0.4 50 D6 2.0 0.5 50
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