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2. What is the effect of enzyme concentration on reaction rate?

2. What is the effect of enzyme concentration on reaction rate?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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2. What is the effect of enzyme concentration on reaction rate?
Transcribed Image Text:2. What is the effect of enzyme concentration on reaction rate?
EFFECT OF ENΖΥΜΕ CONCENTRATIOΝ ON ΕNZΥΜΕ ACTIVITY Introduction: Enzymes are highly specialized class of proteins. They catalyze thousands of chemical reactions with remarkable property of speeding up chemical reactions. They combine with the reactants to produce a transition state having less energy than the transition state of uncatalyzed reaction. When reaction products are formed the free catalyst is regenerated. The rate of enzyme catalyzed reaction is determined by the concentration of the enzyme used. The more concentrated the enzyme used, the faster is the reaction. Objectives: At the end of the activity, the student can: 1. Determine the effect of different concentration of enzyme on reaction rate 2. Compute for the concentration of enzyme present in each tube. 3. Determine in which concentration reaction rate is fastest and slowest. Reagents: Saliva, 6 ml 1% starch solution, 6 drops Iz in KI, 6 ml benedict's reagent. Materials: 12 Test tube (20 ml), 10 ml graduated cylinder, test tube rack, brush, 250 ml beaker, tripod, wire gauze, thermometer, alcohol lamp Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add 1ml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
Transcribed Image Text:EFFECT OF ENΖΥΜΕ CONCENTRATIOΝ ON ΕNZΥΜΕ ACTIVITY Introduction: Enzymes are highly specialized class of proteins. They catalyze thousands of chemical reactions with remarkable property of speeding up chemical reactions. They combine with the reactants to produce a transition state having less energy than the transition state of uncatalyzed reaction. When reaction products are formed the free catalyst is regenerated. The rate of enzyme catalyzed reaction is determined by the concentration of the enzyme used. The more concentrated the enzyme used, the faster is the reaction. Objectives: At the end of the activity, the student can: 1. Determine the effect of different concentration of enzyme on reaction rate 2. Compute for the concentration of enzyme present in each tube. 3. Determine in which concentration reaction rate is fastest and slowest. Reagents: Saliva, 6 ml 1% starch solution, 6 drops Iz in KI, 6 ml benedict's reagent. Materials: 12 Test tube (20 ml), 10 ml graduated cylinder, test tube rack, brush, 250 ml beaker, tripod, wire gauze, thermometer, alcohol lamp Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add 1ml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
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