2. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?

Q: When would you expect to see the E. coli produce the enzyme beta galactosidase if the growth media…

A: In prokaryotes such as E.coli have controlled gene expression by controlling the rate of…

Q: purpose of adding soap to the soil and water

A: Soap are sodium or potassium salt of long chain of fatty acid. It is biodegradable.  Produces scum…

Q: What growth condition would you use to study a psychrotolerant pathogen that contaminates cheese and…

A: Specific media are needed for cell culture depending on the needs of the cells. Following…

Q: What bacteria is growing on this nutrient agar?

A: Microorganisms require nutrients for life, development, and reproduction, which are provided by…

Q: What is the purpose of the crystal violet, bile salts, and neural red in MacConkey Agar media?

A: MacConkey Agar or MAC is a culture medium. It is a selective and differential medium designed for…

Q: Explain the morphology of Bacillus Cereus, Bacillus Subtilis and Bacillus megaterium on a streak…

A: Bacillus cereus, Bacillus subtilis, and Bacillus megaterium are all Gram-positive, aerobic,…

Q: While working with Mannitol Salt Agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar (MSA) is a media which has high concentration of salt solution. There are found…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: Two different strains of the same bacteria grow on minimal media. What would be the outcome of…

A: The many microbial species exhibit a wide range of activities. One of the bacterial groupings is…

Q: What is it called when you stab the nutrient agar during the inoculation step of isolating a…

A: Introduction :  A solid or liquid media that contains nutrients and is used to cultivate bacteria or…

Q: What type pof media encourages the growth of some organisms but suppresses the growth of others?…

A: Culture media are also known as nutritive media or growth media and they are the nutritive mixtures…

Q: while working with mannitol salt agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar is used as a selective and differential medium for the identification and…

Q: Which of the following media are selective, differential, and test for lactose fermenters? What is…

A: MacConkey plates are selective , differential and test for lactose fermenters.

Q: Why do three medium types contain nutrients such as peptone, try prone or soytone?

A: Bacteria, molds and yeast that exists in nature can also be grown in laboratory. Including water,…

Q: What types of bacteria are inhibited on MacConkey agar

A: MacConkey agar,  contains peptones, lactose, bile salts, NaCl, neutral red (pH indicator), and…

Q: What additional information can you get by using selective and differential media that you cannot…

A: Media for microorganism culture consists of the substances that are required for the growth of…

Q: Why are Sterilization, Antisepsis and disinfection necessary?

A: Sterilization is the process of killing or deactivating all forms of microorganisms and spores.…

Q: what  are these bacteria growing on BA and Maconkey agar

A: Blood agar or BA is an enriched culture medium having multiple nutrients. Blood agar is generally…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.