2. In a study, cats were fasted overnight then given a single meal complete in all amino acids except arginine. Within 2 hours, blood ammonia levels increased from a normal level of 94 umol/L to 470 umol/L, and the cats showed the clinical symptoms of ammonia toxicity. A control group fed a complete amino acid diet, or an amino acid diet, in which arginine was replaced by ornithine showed no unusual clinical symptoms. What causcd the ammonia levels to rise in the experimental group? Is arginine an essential amino acid in cats? Why or why not? For the answer: a) point out the role of fasting in the experiment; b) explain, why the absence of arginine can lead to ammonia toxicity; c) explain, the possibility of arginine substitution by ornithine; d) present the particular schemes to confirm your answer. ep

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