2. How would our experimental results change if a less polar mobile phase was used such as butanol instead of isopropyl aleohol?

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1. If we wanted to increase the separation efficiency of the molecules in this experiment, should we
increase or decerease the length of our column? Explain your answer.
2. How would our experimental results change if a less polar mobile phase was used such as
butanol instead of isopropyl alcohol?
3. Suppose three molecules are in a sample: A, B, and C. If the sample was separated using this
experimental procedure, what order would the compounds elute (from first to last)? Molecule A
is a nonpolar compound. Molecule C is a polar compound that also has hydrogen-bonding
intermolecular forces. Molecule B is polar compound without any hydrogen bonding sites.
4.
The following molecules are separated using this experimental technique. List the molecules the
order in which they would elute (first being the first to come off the column, third last).
MOLECULE
LEWIS STRUCTURE
ORDER OF ELUTION
H.
H
.C
Acetone
H.
H
H.
H
C
Pentane
H
H.
H
H.
H
H.
H.
Methylamine
186 |EXPERIMENT 13
86
Transcribed Image Text:1. If we wanted to increase the separation efficiency of the molecules in this experiment, should we increase or decerease the length of our column? Explain your answer. 2. How would our experimental results change if a less polar mobile phase was used such as butanol instead of isopropyl alcohol? 3. Suppose three molecules are in a sample: A, B, and C. If the sample was separated using this experimental procedure, what order would the compounds elute (from first to last)? Molecule A is a nonpolar compound. Molecule C is a polar compound that also has hydrogen-bonding intermolecular forces. Molecule B is polar compound without any hydrogen bonding sites. 4. The following molecules are separated using this experimental technique. List the molecules the order in which they would elute (first being the first to come off the column, third last). MOLECULE LEWIS STRUCTURE ORDER OF ELUTION H. H .C Acetone H. H H. H C Pentane H H. H H. H H. H. Methylamine 186 |EXPERIMENT 13 86
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