2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0 0.0067 1.43 1.6 49.6 0 0.005 1.11
2. Enzyme Kinetics at Different Temperatures During a test of kinetics of an enzyme catalyzed reaction, the following data were recorded: a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor present for temperatures of 30C and 49.6C. b. Use the results from part A to indicate if the reaction is in the temperature activation region when T is increased from 30C to 49.6C. c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being activated at the two temperatures) and the pre-exponential constant. Table 2 Eo T I S V g/L °C mmol/mL mmol/mL mmol/mL-min 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0 0.0067 1.43 1.6 49.6 0 0.005 1.11
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:2. Enzyme Kinetics at Different Temperatures
During a test of kinetics of an enzyme catalyzed reaction, the following data were
recorded:
a. Determine the Michaelis-Menten constant and Vmax for the reaction with no inhibitor
present for temperatures of 30C and 49.6C.
b. Use the results from part A to indicate if the reaction is in the temperature activation
region when T is increased from 30C to 49.6C.
c. Create an Arrhenius plot using the Vmax results for the two temperatures in part A for an
enzyme concentration of 1.6 and estimate the values of Ea (if indeed the enzyme is being
activated at the two temperatures) and the pre-exponential constant.
Table 2
Eo
T
I
S
V
g/L
°C
mmol/mL
mmol/mL
mmol/mL-min
1.6
30
0.1
2.63
1.6
30
0
0.033
1.92
1.6
30
0
0.02
1.47
1.6
30
0
0.01
0.96
1.6
30
0
0.005
0.56
1.6
49.6
0
0.1
5.13
1.6
49.6
0
0.033
3.70
1.6
49.6
0
0.01
1.89
1.6
49.6
0.0067
1.43
1.6
49.6
0
0.005
1.11
Transcribed Image Text:2.
D
A)
VMAX
KM
T=
T=
30C
3.2948
0.0244
49.6 C
6.3411
0.0235
B) Yes, it is in the Activation Temp Area when T =
30C - 49.6C
C). V
=
(Ae-(A) * CE) |
EA
= -49.703
R
Plot
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