2. A protein complex has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to electrophoresis in the presence of SDS only, the complex yields three bands with approximate molecular weights of 180 kDa, 160 kDa, and 60 kDa. When subjected to electrophoresis in the presence of SDS and a reducing agent, it yields three bands with approximate molecular weights of 160 kDa, 90 kDa, and 60 kDa. What is the subunit composition of this protein complex? What type of oligomer is this? What can you say about the interactions that maintain the quaternary structure of the complex?
2. A protein complex has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to electrophoresis in the presence of SDS only, the complex yields three bands with approximate molecular weights of 180 kDa, 160 kDa, and 60 kDa. When subjected to electrophoresis in the presence of SDS and a reducing agent, it yields three bands with approximate molecular weights of 160 kDa, 90 kDa, and 60 kDa. What is the subunit composition of this protein complex? What type of oligomer is this? What can you say about the interactions that maintain the quaternary structure of the complex?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Transcribed Image Text:2. A protein complex has a molecular mass of 400 kDa when measured by size-exclusion chromatography.
When subjected to electrophoresis in the presence of SDS only, the complex yields three bands with
approximate molecular weights of 180 kDa, 160 kDa, and 60 kDa. When subjected to electrophoresis in
the presence of SDS and a reducing agent, it yields three bands with approximate molecular weights of
160 kDa, 90 kDa, and 60 kDa. What is the subunit composition of this protein complex? What type of
oligomer is this? What can you say about the interactions that maintain the quaternary structure of the
complex?
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