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- 10.) Draw a double-stranded DNA molecule (using different colors for each) model should clearly represent the sequence: A G T A C C G G G C A A Note: It should include items to represent - sugar molecules - phosphate molecules - 4 distinct nitrogenous base molecules (Adenine, Thymine, Cytosine, and Guanine) - two types of bonds between these molecules13. a. Draw in the corresponding DNA nucleotide that would base pair with the adenine nucleotide shown below using the same level of detail as provided in the image below. Hint: Remember that DNA bases pair in the antiparallel orientation. орасна you A OH H b. How do you know the adenine nucleotide drawn above is from DNA and not RNA?32. The molecule above is called HO 08-0 OH OH and it can be found in A) 3'-deoxyribose-5'-phosphate; RNA B) 2'-deoxyribose-5'-phosphate; RNA 3'-deoxyribose-5'-phosphate; DNA D) 2'-deoxyribose-5'-phosphate; DNA 33. The chromosomes we typically con in im OH
- 5' C C A U G A C G U C G G A U C A A U G A G C 3' 2. If the nucleotide bolded and underlined in red in part 1 changes from C to a G, what type of mutation would that be? 3. What would happen to the amino acid sequence if the C bolded in red in part 1 is changed to a G? 4. What would happen to the amino acid sequence if the C bolded in red is deleted?Are both statements true? 1. Heterogeneous RNA is a term that refers to mRNA that has not been processed. 2. If the %A of a bacteria is 20%, the amount of guanine is 30%. Are both statements true? 1. If the %A of a bacteria is 30%, then the % (G+C) of the bacteria is 40% 2. The DNA is plectonemic because one of the strands go in 5' to 3' direction while the other go in the 3 to 5 direction17. Mutation can be caused by the alternative base pairing that arise due to tautomerization and deamination of cysteine and adenine. Draw the alternative base pairing. The thymine dimers can be formed by UV radiation. Alternate base pairing with enol tautomer of T Alternate base pair with deaminated A Thymine dimers due to UV radn (draw structure below) repaired by the enzyme Methylated cytosine and adenine in mismatch repair
- 6. a.) Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? b.) Based on the complementary base pairing rules we know that: A(denosine) pairs with _________ , and that G(uanine) pairs with _________.21. The difference between a nucleoside and nucleotide is: Group of answer choices 1. A nucleoside consists of the sugar with a nitrogenous base, whereas a nucleotide has a sugar with a nitrogenous base and phosphate groups attached to the sugar. 2. Nucleotides contain deoxyribose sugar and nucleosides contain ribose sugar 3. Nucleotides are involved in eukaryotic DNA replication, while nucleosides are used in bacterial DNA replication 4. A nucleotide consists of the sugar with a nitrogenous base, whereas a nucleoside has a sugar with a nitrogenous base and phosphate groups attached to the sugar.13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the primers bind, what occurs next? 16. What does "highly conserved" mean? 17. Why are highly conserved regions important in this lab?
- 4. Determine the answers of these multiple choices they are organised at "a,b,c's" in which is allowed in your rules, so they can all be answered thank you very much!! A) A DNA nucleotide is composed of: *Nitrogen base *Phosphate - ribose - nitrogen base *Ribose - nitrogen base *Phosphate - deoxyribose - nitrogen base *Deoxyribose - nitrogen base B) An amino acid contains the following two groups: Acid and Thiol -Carboxyle and Amine -Carbonyl and Carboxyl -Thiol and Amine -Amine and Acid C) Which carbohydrate is part of an RNA molecule? -Ribose -Galactose -Fructose -Glucose -Deoxyribose1. Which of the following RNA has a catalytic activity like an enzyme? A. mRNA C. rRNA B. tRNA D. micro RNA 2.What is the correct name of a nucleotide with the following constituents: Thymine + D- ribose + PO4? A. Deoxythymidine 5'-monophosphate B. Thymidine 5'-monophosphate 3.What is the difference between A DNA and B DNA? C. Ribonucleothymidine 3'-monophosphate D. This nucleotide doesn't exist. A. They differ in orientation B. They differ in pitch 4. Following the complementary base pairing rule, predict the percentage of T in a double stranded DNA with 17% of C. A. 25% B. 30% 5.If Guanine combines with deoxyribose, what compound is formed? C. Guanosine D. Guanine 3'-monophosphate A. Deoxyguanine 5'monophosphate B. Deoxyguanosine C. They differ in direction of coiling D. B and C C. 33% D. 35%(True or False) 1.) Fatty acids can be found in ceramides and TAGs. 2.) The adenine base can easily form hydrogen bonds with thymine with the least energy requirement. 3.) The 3'end of nucleic acids structurally has a free OH group bonded to the 3' carbon of the sugar. 4.) Naturally occurring RNA molecules are always single stranded in the cell 5.) The Watson and Crick double stranded DNA structure is always antiparallel.