Homework Help is Here – Start Your Trial Now!
Science
Biology
16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so...Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis - bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min?

16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so...Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis - bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
Need help with the questions in yellow highlight
The data presented below was from an actual experiment performed by Bio 102 students. Competitive inhibition was assessed on PEROXIDASE, an enzyme found in turnips. Peroxidase works on H2O2 to detoxify the hydrogen peroxide by generating water and molecular oxygen as the products. Guaiacol binds with oxygen to yield a brown coloration that can be detected with a spectrophotometer. In other words, one of the products can be detected and measured. The greater the amount of O2, the more intense the coloration, the greater the absorbance at 470nm. Hydroxylamine has a shape similar to H2O2. Evaluate the tube conditions in the first table and correlate that with the spec results in the second table. Answer the questions below the tables. Table 11.5 Experimental Conditions to Test the Inhibition of Hydroxylamine on Peroxidase Activity Hydrogen Peroxide (3%) Distilled Guaiacol (25 mM) Turnip Extract Hydroxylamine (10%) Tube Water 5.9 ml. 0.1 ml 2 5.8 ml. 0.2 ml. 3 5.7 ml. 0.1 ml. 0.2 ml. 4.9 ml. 0.1 ml 1.0 ml. 4,7 ml. 0.1 ml. 0.2 ml. 1.0 ml. 6. 4.2 ral. 0.1 ml 0.2 ml. 1.0 ml. 0.5 ml. 3.7 ml. 0.1 ml. 0.2 ml. 15 ml. 05 ml. 3.2 al. 0,1 ml. 0.2 ml. 2.0 ml. 05 ml. 2.2 ml. 0.1 ml. 0.2 ml. 3.0 ml. 05 ml. 2 |Page Table 11.6 Absorbance at 470 nm of Peroxide/Peroxidase Solutions 0.0 0.5 min 1.0 1.5 min 2.0 min 2.5 min 3.0 3.5 4.0 4.5 min 5.0 min Tube min min min min min 0.0j2 0.D100.0I0 0.010 0.010 0.010 0.01d o-0j0l o.01ol0.010 0.010 0,008 0.008 0-008 0-008 o.008 0.008 o.008 0008 0-008 o.008 0.008 0:01le 0.01i 0.01) 0.011 0.DII 0.DI1 0.011 0,DIl OI011 0-011 o.01 l0:0640.0590:058 0,058 0,059 0.059 0059|0:059 0.059 0.0590.059 1.999 |1.999 | 1.999 |1.999|1999|1L999 1,999|1999|1999 1.999|1999 0.132 0.203 0,288 0,340 0140001434 0.450014600.46201454 01488 l0.448/0.688D.950 1,170 1,3301.500 1,6001,70117801.852 1910 0.378 0.778 1,130 1.420 1.660 1.850 1.999 1.9991.999 1.999 1.999 0.825 1,5601.999 1.999 1.999 1991.99¶|19991.999 1.999 1.999 4 8 13)Write a null hypothesis and an alternate hypothesis Ter the impact of the inhibitor en enzyme aetivity: 14) Summary: What are the fellowing eompunents in the experiment? Substrate Ar- Enzyme C.Inhibitor . Indieator 15) Leek at the esults for tubes +4. Explain the results that were obtained for these tubes. 16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so....Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis – bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min? 19) Could you still detect peroxidase activity in the presence of the inhibitor? Why? 20) Where do you think the inhibitor had its impact? Why is it able to do what it has done? Hint: I tell you above the tables. How do organisms exploit the use of inhibitors?
Transcribed Image Text:The data presented below was from an actual experiment performed by Bio 102 students. Competitive inhibition was assessed on PEROXIDASE, an enzyme found in turnips. Peroxidase works on H2O2 to detoxify the hydrogen peroxide by generating water and molecular oxygen as the products. Guaiacol binds with oxygen to yield a brown coloration that can be detected with a spectrophotometer. In other words, one of the products can be detected and measured. The greater the amount of O2, the more intense the coloration, the greater the absorbance at 470nm. Hydroxylamine has a shape similar to H2O2. Evaluate the tube conditions in the first table and correlate that with the spec results in the second table. Answer the questions below the tables. Table 11.5 Experimental Conditions to Test the Inhibition of Hydroxylamine on Peroxidase Activity Hydrogen Peroxide (3%) Distilled Guaiacol (25 mM) Turnip Extract Hydroxylamine (10%) Tube Water 5.9 ml. 0.1 ml 2 5.8 ml. 0.2 ml. 3 5.7 ml. 0.1 ml. 0.2 ml. 4.9 ml. 0.1 ml 1.0 ml. 4,7 ml. 0.1 ml. 0.2 ml. 1.0 ml. 6. 4.2 ral. 0.1 ml 0.2 ml. 1.0 ml. 0.5 ml. 3.7 ml. 0.1 ml. 0.2 ml. 15 ml. 05 ml. 3.2 al. 0,1 ml. 0.2 ml. 2.0 ml. 05 ml. 2.2 ml. 0.1 ml. 0.2 ml. 3.0 ml. 05 ml. 2 |Page Table 11.6 Absorbance at 470 nm of Peroxide/Peroxidase Solutions 0.0 0.5 min 1.0 1.5 min 2.0 min 2.5 min 3.0 3.5 4.0 4.5 min 5.0 min Tube min min min min min 0.0j2 0.D100.0I0 0.010 0.010 0.010 0.01d o-0j0l o.01ol0.010 0.010 0,008 0.008 0-008 0-008 o.008 0.008 o.008 0008 0-008 o.008 0.008 0:01le 0.01i 0.01) 0.011 0.DII 0.DI1 0.011 0,DIl OI011 0-011 o.01 l0:0640.0590:058 0,058 0,059 0.059 0059|0:059 0.059 0.0590.059 1.999 |1.999 | 1.999 |1.999|1999|1L999 1,999|1999|1999 1.999|1999 0.132 0.203 0,288 0,340 0140001434 0.450014600.46201454 01488 l0.448/0.688D.950 1,170 1,3301.500 1,6001,70117801.852 1910 0.378 0.778 1,130 1.420 1.660 1.850 1.999 1.9991.999 1.999 1.999 0.825 1,5601.999 1.999 1.999 1991.99¶|19991.999 1.999 1.999 4 8 13)Write a null hypothesis and an alternate hypothesis Ter the impact of the inhibitor en enzyme aetivity: 14) Summary: What are the fellowing eompunents in the experiment? Substrate Ar- Enzyme C.Inhibitor . Indieator 15) Leek at the esults for tubes +4. Explain the results that were obtained for these tubes. 16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so....Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis – bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min? 19) Could you still detect peroxidase activity in the presence of the inhibitor? Why? 20) Where do you think the inhibitor had its impact? Why is it able to do what it has done? Hint: I tell you above the tables. How do organisms exploit the use of inhibitors?
Nyll Hy pothesis :There is no change in enzyme activity of peroxidase with change in innibitor concentration. Atternate Hypotnesis: Enzyme activity dcreases witn increase in inhibitor concentration, witn time. Summary: Components of the reaction a) SubStrate : Hydrogen peroxide, HyDz 6) Enzy me : Peroxidase c) Inhibitor: Hydroxylamine dl Indicator: Guaiacol Look at the veSult for tubes F4.Explain the vesults that were obtained for +hese tubes In tubes 1,2,3 and 4, the en yme activity 1s constant witn time. The initial vate of the experiment is constant because there iS the pressence Of a hvge amount of Substrate. Initially, the fow enzyme molecules work an lange quantities of Substrates and the initial and final Substrate concentration vemains the Same. The enzyme activity is the same for 5 min.
Transcribed Image Text:Nyll Hy pothesis :There is no change in enzyme activity of peroxidase with change in innibitor concentration. Atternate Hypotnesis: Enzyme activity dcreases witn increase in inhibitor concentration, witn time. Summary: Components of the reaction a) SubStrate : Hydrogen peroxide, HyDz 6) Enzy me : Peroxidase c) Inhibitor: Hydroxylamine dl Indicator: Guaiacol Look at the veSult for tubes F4.Explain the vesults that were obtained for +hese tubes In tubes 1,2,3 and 4, the en yme activity 1s constant witn time. The initial vate of the experiment is constant because there iS the pressence Of a hvge amount of Substrate. Initially, the fow enzyme molecules work an lange quantities of Substrates and the initial and final Substrate concentration vemains the Same. The enzyme activity is the same for 5 min.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Environmental health & hazards
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS