Need help with the questions in yellow highlight The data presented below was from an actual experiment performed by Bio 102 students.Competitive inhibition was assessed on PEROXIDASE, an enzyme found in turnips. Peroxidaseworks on H2O2 to detoxify the hydrogen peroxide by generating water and molecular oxygen asthe products. Guaiacol binds with oxygen to yield a brown coloration that can be detected with aspectrophotometer. In other words, one of the products can be detected and measured. Thegreater the amount of O2, the more intense the coloration, the greater the absorbance at 470nm.Hydroxylamine has a shape similar to H2O2. Evaluate the tube conditions in the first table andcorrelate that with the spec results in the second table. Answer the questions below the tables.Table 11.5Experimental Conditions to Test the Inhibition of Hydroxylamine on Peroxidase ActivityHydrogenPeroxide(3%)DistilledGuaiacol(25 mM)TurnipExtractHydroxylamine(10%)TubeWater5.9 ml.0.1 ml25.8 ml.0.2 ml.35.7 ml.0.1 ml.0.2 ml.4.9 ml.0.1 ml1.0 ml.4,7 ml.0.1 ml.0.2 ml.1.0 ml.6.4.2 ral.0.1 ml0.2 ml.1.0 ml.0.5 ml.3.7 ml.0.1 ml.0.2 ml.15 ml.05 ml.3.2 al.0,1 ml.0.2 ml.2.0 ml.05 ml.2.2 ml.0.1 ml.0.2 ml.3.0 ml.05 ml.2 |PageTable 11.6Absorbance at 470 nm of Peroxide/Peroxidase Solutions0.00.5min1.01.5min2.0min2.5min3.03.54.04.5min5.0minTubeminminminminmin0.0j2 0.D100.0I0 0.010 0.010 0.010 0.01d o-0j0l o.01ol0.010 0.0100,008 0.008 0-008 0-008 o.008 0.008 o.008 0008 0-008 o.008 0.0080:01le 0.01i 0.01) 0.011 0.DII 0.DI1 0.011 0,DIl OI011 0-011 o.01l0:0640.0590:058 0,058 0,059 0.059 0059|0:059 0.059 0.0590.0591.999 |1.999 | 1.999 |1.999|1999|1L999 1,999|1999|1999 1.999|19990.132 0.203 0,288 0,340 0140001434 0.450014600.46201454 01488l0.448/0.688D.950 1,170 1,3301.500 1,6001,70117801.852 19100.378 0.778 1,130 1.420 1.660 1.850 1.999 1.9991.999 1.999 1.9990.825 1,5601.999 1.999 1.999 1991.99¶|19991.999 1.999 1.9994813)Write a null hypothesis and an alternate hypothesis Ter the impact of the inhibitor enenzyme aetivity:14) Summary: What are the fellowing eompunents in the experiment?SubstrateAr-EnzymeC.Inhibitor. Indieator15) Leek at the esults for tubes +4. Explain the results that were obtained for these tubes.16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or ifyou choose to plot with Excel (high tech), then you may do so....Enzyme Activity(Absorbance) is on one axis and Time is on the other (you select which is the y-axis andwhich is the x-axis – bear in mind which is the dependent variable and which is theindependent variable).17) What conclusion can you draw about the way the graph looks?18) Which tubes show peroxidase activity after 5 min?19) Could you still detect peroxidase activity in the presence of the inhibitor? Why?20) Where do you think the inhibitor had its impact? Why is it able to do what it has done?Hint: I tell you above the tables.How do organisms exploit the use of inhibitors? Nyll Hy pothesis :There is no change in enzyme activity of peroxidase with change in innibitor concentration.Atternate Hypotnesis: Enzyme activity dcreases witn increase ininhibitor concentration, witn time.Summary: Components of the reactiona) SubStrate : Hydrogen peroxide, HyDz6) Enzy me : Peroxidasec) Inhibitor: Hydroxylaminedl Indicator: GuaiacolLook at the veSult for tubes F4.Explain the vesults that were obtained for +hese tubesIn tubes 1,2,3 and 4, the en yme activity 1s constant witn time. The initial vate of the experiment is constant because there iS the pressenceOf a hvgeamount of Substrate. Initially, the fow enzyme molecules work an lange quantities of Substratesand the initial and final Substrate concentration vemains the Same.The enzyme activity is the same for 5 min.

Note: According to the given guidelines, we are supposed to answer only 3 subparts of the given…

16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so...Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis - bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min?

16) Look at the results for tubes 5-9. Plot these on the graph paper provided (low tech) or if you choose to plot with Excel (high tech), then you may do so...Enzyme Activity (Absorbance) is on one axis and Time is on the other (you select which is the y-axis and which is the x-axis - bear in mind which is the dependent variable and which is the independent variable). 17) What conclusion can you draw about the way the graph looks? 18) Which tubes show peroxidase activity after 5 min?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 46SST

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