Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
1.The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: (nm/mn) Unitsi Km > mm) Substrate Concentration units: Vmas > Velocity (nanomoles/min) No inhibitor in millimoles (mM) With inhibitor 55.8 x 10 54.9 x 10 51 x 10 47 x 10 40 x 10' 28 x 10 18.7 x 10 11.2 x 10 2.7 x 10 55.7 x 10 53.3 x 103 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested o.2 05 S. 2. A. What are the Vmax and Km values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km B. (i). What are the apparent Vmax and Km Values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know?

1.The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: (nm/mn) Unitsi Km > mm) Substrate Concentration units: Vmas > Velocity (nanomoles/min) No inhibitor in millimoles (mM) With inhibitor 55.8 x 10 54.9 x 10 51 x 10 47 x 10 40 x 10' 28 x 10 18.7 x 10 11.2 x 10 2.7 x 10 55.7 x 10 53.3 x 103 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested o.2 05 S. 2. A. What are the Vmax and Km values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km B. (i). What are the apparent Vmax and Km Values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Please complete Boothe parts of part B thoroughly with detailed explanations since only part A was done already. Thank you
ENZYME KINETICS; PATHWAYS & FEEDBACK INHIBITION 3-1. The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: UnitsiKm> (mm) units: Vmon > (nm/mn) Velocity (nanomoles/min) No inhibitor 55.8 x 103 54.9 x 10 51 x 10 47 x 10 40 x 103 28 x 10 18.7 x 10 11.2 x 103 2.7 x 10° Substrate Concentration in millimoles (mM) With inhibitor 55.7 x 10 53.3 x 10 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested 0.2 0°5 2. A. What are the Vmax and Km Values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km → B. (i). What are the apparent Vmax and Km values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know? Note: You do not need a calculator or graph paper to figure any of this out. However you may want to plot the results on graph paper to get a better feel for what is going on. If you do it that way, try to do it again without the graph, using the numbers shown.
Transcribed Image Text:ENZYME KINETICS; PATHWAYS & FEEDBACK INHIBITION 3-1. The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: UnitsiKm> (mm) units: Vmon > (nm/mn) Velocity (nanomoles/min) No inhibitor 55.8 x 103 54.9 x 10 51 x 10 47 x 10 40 x 103 28 x 10 18.7 x 10 11.2 x 103 2.7 x 10° Substrate Concentration in millimoles (mM) With inhibitor 55.7 x 10 53.3 x 10 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested 0.2 0°5 2. A. What are the Vmax and Km Values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km → B. (i). What are the apparent Vmax and Km values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know? Note: You do not need a calculator or graph paper to figure any of this out. However you may want to plot the results on graph paper to get a better feel for what is going on. If you do it that way, try to do it again without the graph, using the numbers shown.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Medical terminologies
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS