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1. What does the acronym ELISA mean? 2. Of the different types of biological macromolecules, which ones most commonly serve as antigens to which antibodies will bind? 3. Describe the roles of the 1st Antibody and the 2nd Antibody during an ELISA test. 4. Diagram a well containing all components necessary for a positive ELISA result. 5. After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound to 1st Antibody bound to 2nd Antibody? а. 6. Describe how you could use ELISA to determine if the human and cow versions of a specific protein(e.g. the blood protein albumin) have a similar epitope. 7. Explain at least one way you could have a false positive, and a false negative in the HHMI ELISA for SLE. 8. In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?

1. What does the acronym ELISA mean? 2. Of the different types of biological macromolecules, which ones most commonly serve as antigens to which antibodies will bind? 3. Describe the roles of the 1st Antibody and the 2nd Antibody during an ELISA test. 4. Diagram a well containing all components necessary for a positive ELISA result. 5. After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound to 1st Antibody bound to 2nd Antibody? а. 6. Describe how you could use ELISA to determine if the human and cow versions of a specific protein(e.g. the blood protein albumin) have a similar epitope. 7. Explain at least one way you could have a false positive, and a false negative in the HHMI ELISA for SLE. 8. In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. What does the acronym ELISA mean? 2. Of the different types of biological macromolecules, which ones most commonly serve as antigens to which antibodies will bind? 3. Describe the roles of the 1st Antibody and the 2nd Antibody during an ELISA test. 4. Diagram a well containing all components necessary for a positive ELISA result. 5. After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound to 1st Antibody bound to 2nd Antibody? а. 6. Describe how you could use ELISA to determine if the human and cow versions of a specific protein(e.g. the blood protein albumin) have a similar epitope. 7. Explain at least one way you could have a false positive, and a false negative in the HHMI ELISA for SLE. 8. In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?
Transcribed Image Text:1. What does the acronym ELISA mean? 2. Of the different types of biological macromolecules, which ones most commonly serve as antigens to which antibodies will bind? 3. Describe the roles of the 1st Antibody and the 2nd Antibody during an ELISA test. 4. Diagram a well containing all components necessary for a positive ELISA result. 5. After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound to 1st Antibody bound to 2nd Antibody? а. 6. Describe how you could use ELISA to determine if the human and cow versions of a specific protein(e.g. the blood protein albumin) have a similar epitope. 7. Explain at least one way you could have a false positive, and a false negative in the HHMI ELISA for SLE. 8. In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the difference between the presence of IgG and IgM in a sample?
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