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- What is application of D_serine?Arial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000Explain the mechanism action of denaturation of protein from egg (Albumin) using isopropyl alcohol.
- I. A protein, X, was Isolated from a pathogenlc mlcroorganism. The proteln Is a vlrulence factor whose path0genlclty lies In a heptapeptide of unknown sequence. After trypsin cleavage of the heptapeptide from protein X, the peptlde's compOsition and sequence was determined. The fOllowing were the results of the sequenclng process: 1. When the peptide was treated with dinitrofluorobenzene (DNFB), DNP-asp and a mixture of amino acids were produced. 2. When the same Intact peptide was treated with streptococcal protease, a pentapeptide of composition asp, asN, cys, gly and ser and 2 amlno acids were released. 3. When the heptapeptlde was also treated with hydrOxylamine HCI, a tripeptide and a tetrapeptide were obtained. The C-terminal amino acid of the tripeptide was asN. 1) What is the sequence of the heptapeptide if it is composed of cys, asp, lys, asN, gly and ser only? 2) What is the pl of the heptapeptide?4 A I. Refer to the figure below and answer the following questions: 45 5 55 6 65 PH 7 75 8.5 0 10 B 15 20 25 30 35 Temperature (°C) 40 45 Legend: Blue - wild-type ß-galactosidase; Red - mutant ß-galactosidase a. What is the optimum pH of wild type ß-galactosidase? b. What is the optimum temperature of mutant ß-galactosidase? c. Which enzyme has the greater activity at pH 7.2? d. Which enzyme has the greater activity at a temperature of 42.5°C? e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4? f. Which enzyme has greater activity if temperature increases from 40°C to 41 °C? 50 5539. Which ONE of the following action would be most selectively toxic against bacterial cell? Group of answer choices inhibiting DNA replication preventing cell wall synthesis interrupting peptidyl transferase functions targeting codon : anticodon interaction disturbing cell membrane integrity 40. Identify the TRUE statement from the following, Group of answer choices superantigens are made by our body’s cell superantigens inhbits cytokines release Bacillus anthracis secreted toxin is a superantigen Staphylococcus toxic shock syndrome toxin is a superantigen superantigens activates B cells of defense system
- Non-enzymatic browning Proteins: Lysin, methionine Sugars: glucose, galactose, sucrose, fructose, maltose 1. Describe (the aromas produced) and the degree of browning for each solution 2. Explain variations in color with respect to each sugar-amino acid combination by taking the chemical structures of sugars and amino acids into considerationWhat is the name of the fungal toxin that inhibits RNA polymerase Il at 1 microgram/mL concentration? O Flavopiridol a-Amanitin O Triptolide DRB (5,6-Dichloro-1-ß-D- ribofuranosylbenzimidazole)15 Explain why detergents, which have an amphipathic structure like bile salts, are able to remove both water-based and oil-based stains.
- 1. What is the biochemical pathway for tryptophan? A：Precursor-----AA---tryptophan------I------GP B：Precursor-----AA--------GP---------I--------tryptophan C：Precursor---AA-------I---------GP--------tryptophan D：precursor------GP------AA------I---------tryptophan E：Precursor------I--------AA---------GP-----tryptophan 2. Mutant trp11 is？ A：blocking first step of the pathway B：blocking second step of the pathway C：blocking third step of the pathway D：blocking fourth or final step of the pathwayB W S sult, it makes the protein Why is denaturing the proteins necessary for SDS PAGE to work? Use YOUR OWN X mand 3 E D с $ R F 5 Lê 6 T G MacBook Pro B Y H U N J 8 I M 9 K O I O L 44 P command . : 8 ; x { + C option ? I } 1 MWhich letter represents the target site of furosemide (Lasix)? E- -B