1. The kcat of an enzymatic reaction depends on________________ (Circle two answers!) A. The free energy change (ΔG) of the chemical reaction B. The substrate concentration C. The activation energy ΔG‡ of the chemical reaction D. The enzyme concentration E. The temperature
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
1. The kcat of an enzymatic reaction depends on________________
(Circle two answers!)
A. The free energy change (ΔG) of the
B. The substrate concentration
C. The activation energy ΔG‡ of the chemical reaction
D. The enzyme concentration
E. The temperature
2. The KM of an enzymatic reaction depends on______________________
(Circle two answers!)
A. The free energy change (ΔG) of the chemical reaction
B. The substrate
C. The activation energy ΔG‡ of the chemical reaction
D. The enzyme
E. The rate constant, kcat
3. Name three different ways the ‘active site’ of an enzyme can contribute to catalysis
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