1. The initial rate of reaction for the enzymatic cleavage of deoxyguanosine triphosphate was measured as a function of initial substrate concentration as follows Substrate Concentration Initial Reaction Rate umol/L umol/L min 6.7 0.30 3.5 0.25 1.7 0.16 a. Calculate the Michele-Menten constants of the above reaction b. When the inhibitor was added, the initial reaction rate was decreased as follows: Substrate Inhibitor Initial Reaction Rate µmoVL umol/L umoVL min 6.7 146 0.11 3.5 146 0.08 1.7 146 0.06 Is this competitive inhibition or noncompetitive inhibition? Justify your answer by showing the effect of the Inhibitor graphically.
1. The initial rate of reaction for the enzymatic cleavage of deoxyguanosine triphosphate was measured as a function of initial substrate concentration as follows Substrate Concentration Initial Reaction Rate umol/L umol/L min 6.7 0.30 3.5 0.25 1.7 0.16 a. Calculate the Michele-Menten constants of the above reaction b. When the inhibitor was added, the initial reaction rate was decreased as follows: Substrate Inhibitor Initial Reaction Rate µmoVL umol/L umoVL min 6.7 146 0.11 3.5 146 0.08 1.7 146 0.06 Is this competitive inhibition or noncompetitive inhibition? Justify your answer by showing the effect of the Inhibitor graphically.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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1. The initial rate of reaction for the enzymatic cleavage of deoxyguanosine triphosphate was
measured as a function of initial substrate concentration as follows
Substrate Concentration
Initial Reaclion Rate
umol/L
umol/L min
6.7
0.30
3.5
0.25
1.7
0.16
а.
Calculate the Michele-Menten constants of the above reaction
b. When the inhibitor was added, the initial reaction rate was decreased as follows:
Substrate
Inhibitor
Initial Reaction Rate
umoVL
umol/L
umol/L min
6.7
146
0.11
3.5
146
0.08
1.7
146
0.06
Is this competitive inhibition or noncompetitive inhibition? Justify your answer by showing the
effect of the Inhibitor graphically.
2. Assume that experiment for certain microorganism have shown that cells can convert (2/3)
(wt/wt) of the substrate carbon (alkane or glucose) to biomass
23°C
07:47
w
Berawan
26/04/2022
..
>"
Transcribed Image Text:b Success Confirmatic X
O SSO - Dashboard
A Dasar-dasar Biopro X
9 (5) WhatsApp
O YouTube
Google Translate
x +
->
A drive.google.com/drive/folders/10_DzC9YxnUNJnuvolJj-x0awSTUSklTz
1. The initial rate of reaction for the enzymatic cleavage of deoxyguanosine triphosphate was
measured as a function of initial substrate concentration as follows
Substrate Concentration
Initial Reaclion Rate
umol/L
umol/L min
6.7
0.30
3.5
0.25
1.7
0.16
а.
Calculate the Michele-Menten constants of the above reaction
b. When the inhibitor was added, the initial reaction rate was decreased as follows:
Substrate
Inhibitor
Initial Reaction Rate
umoVL
umol/L
umol/L min
6.7
146
0.11
3.5
146
0.08
1.7
146
0.06
Is this competitive inhibition or noncompetitive inhibition? Justify your answer by showing the
effect of the Inhibitor graphically.
2. Assume that experiment for certain microorganism have shown that cells can convert (2/3)
(wt/wt) of the substrate carbon (alkane or glucose) to biomass
23°C
07:47
w
Berawan
26/04/2022
..
>
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