1. The growth of baker's yeast (S. cerevisiae) on glucose may be simply described by the following equation: C6H12O6 + 3 O2 + 0.48 NH3 → 0.48 C6H10NO3 + 4.32 H2O + 3.12 CO2 уeast In a batch reactor of volume 105 1, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry: a) Determine the concentration and total amount of glucose and (NH4)2SO4 in the nutrient medium. b) Determine the yield coefficients Yx/s (biomass/glucose) and Yxo2 (biomass/oxygen). Determine the total amount of oxygen required. c) d) If the rate of growth at exponential phase is rx=0.7 gdw/l-h, determine the rate of oxygen consumption (g O2/1-h).
1. The growth of baker's yeast (S. cerevisiae) on glucose may be simply described by the following equation: C6H12O6 + 3 O2 + 0.48 NH3 → 0.48 C6H10NO3 + 4.32 H2O + 3.12 CO2 уeast In a batch reactor of volume 105 1, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry: a) Determine the concentration and total amount of glucose and (NH4)2SO4 in the nutrient medium. b) Determine the yield coefficients Yx/s (biomass/glucose) and Yxo2 (biomass/oxygen). Determine the total amount of oxygen required. c) d) If the rate of growth at exponential phase is rx=0.7 gdw/l-h, determine the rate of oxygen consumption (g O2/1-h).
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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1.
The growth of baker's yeast (S. cerevisiae) on glucose may be simply described by the following equation:
C6H1206 + 3 O2+ 0.48 NH3 → 0.48 C6H10NO3 + 4.32 H2O + 3.12 CO2
yeast
In a batch reactor of volume 10s 1, the final desired yeast concentration is 50 gdw/l. Using the above reaction
stoichiometry:
a) Determine the concentration and total amount of glucose and (NH4)2SO4 in the nutrient medium.
b) Determine the yield coefficients Yx/s (biomass/glucose) and Yxo2 (biomass/oxygen).
c) Determine the total amount of oxygen required.
d) If the rate of growth at exponential phase is rx= 0.7 gdw/l-h, determine the rate of oxygen consumption
(g O2/l-h).
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The
recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is
synthesised in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration
of glucose.
The recombinant protein has an overall formula of CH1.55O0.31NO.25. The yield of biomass (excluding recombinant
protein) from glucose is measured as 0.48 g g21; the yield of recombinant protein from glucose is about 20% of that
for cells.
2.
How much ammonia is requires
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