1. The concentration of alginate used to immobilize the enzyme was 3% (w/v). What do you think would be the effect of significantly increasing the concentration of alginate used?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. The concentration of alginate used to immobilize the enzyme was 3% (w/v). What do
you think would be the effect of significantly increasing the concentration of alginate
used?
2. What would be the likely effect of significantly speeding up the flow rate of the
sucrose solution through the column?
3. How do you think you could experimentally determine the optimum flow rate through
the column?
4. The substrate (i.e. 3% sucrose is made up in buffer at pH 4.7. If the sucrose was made
up in water at pH 7 the rate of catalytic conversion would be slower. Why?
Transcribed Image Text:Questions. 1. The concentration of alginate used to immobilize the enzyme was 3% (w/v). What do you think would be the effect of significantly increasing the concentration of alginate used? 2. What would be the likely effect of significantly speeding up the flow rate of the sucrose solution through the column? 3. How do you think you could experimentally determine the optimum flow rate through the column? 4. The substrate (i.e. 3% sucrose is made up in buffer at pH 4.7. If the sucrose was made up in water at pH 7 the rate of catalytic conversion would be slower. Why?
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