1. Imagine you are a student in Alfred Hershey and Martha Chase's lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forgot to mark the tubes and are uncertain about which were labeled with ³2P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained E. coli infected with ³2P- labeled phage? Explain your answer. Tube number 1 2 3 4 5 Radioactivity present in Cells Protein coats Protein coats Cells Cells

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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1. Imagine you are a student in Alfred Hershey and Martha Chase's lab in the late 1940s.
You are given five test tubes containing E. coli bacteria infected with T2
bacteriophages that have been labeled with either ³2P or ³5S. Unfortunately, you
forgot to mark the tubes and are uncertain about which were labeled with ³2P and
which with ³5S. You place the contents of each tube in a blender and turn it on for a
few seconds to shear off the phage protein coats. You then centrifuge the contents to
separate the protein coats and the cells. You check for the presence of radioactivity
and obtain the following results. Which tubes contained E. coli infected with ³2P-
labeled phage? Explain your answer.
Tube number
1
2
3
4
5
Radioactivity present in
Cells
Protein coats
Protein coats
Cells
Cells
Transcribed Image Text:1. Imagine you are a student in Alfred Hershey and Martha Chase's lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either ³2P or ³5S. Unfortunately, you forgot to mark the tubes and are uncertain about which were labeled with ³2P and which with ³5S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained E. coli infected with ³2P- labeled phage? Explain your answer. Tube number 1 2 3 4 5 Radioactivity present in Cells Protein coats Protein coats Cells Cells
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