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- adenine molecule ii. NH2 N- НО-Р—О—Р—О—Р—О. ОН ОН ОН i. Phosphate chain ОН ОН iii ribose sugar1. Based on the graph, which of the following could be used to increase the reaction rate beyond point C? a. increase the amount of substrate b. decrease enzyme concentration C. add more water d. increase the temp 2. Which explanation best fits the graph for pH's effect on an enzyme? Substrate concentration a.As the pH gets closer to neutral more H-Bonds are broken b.Larger numbers of H+ & OH- ions distort the active site c. There are more collisions between substrates and active sites near the optimal pH d.Low pHs cause more H-Bonds to happen near the active site Optim pll6. Define: Energy of activation (EA) and tell how enzymes change it. 7. Identify a, b, c, d and e for the Reactions A+B C+D Or C+D A+B C. b. A+B a. е. a. d. C. C+D d. Progress of the Reaction e. Free Energy b.
- 3. a. In the process of converting ADP to ATP, water is circle one: (REQUIRED / RELEASED) and therefore this is called a reaction type. Does this type of reaction require an enzyme? circle one: ( NO / YES / sometimes ) This reaction circle one: (REQUIRES / RELEASES) energy! b. In the process of converting ATP to ADP, water is circle one: (REQUIRED / RELEASED) and therefore this is called a reaction type. Does this type of reaction require an enzyme? circle one: ( NO / YES / sometimes ) This reaction circle one: (REQUIRES / RELEASES) energy! c. Explain why ATP is like a rechargeable battery.7. Which of the following statements is true about enzyme-catalyzed reactions? The reaction is faster than the same reaction in the absence of the enzyme b. The free energy change of the reaction is opposite from the reaction without the enzyme The reaction always goes in the direction toward chemical equilibrium d. Enzyme-catalyzed reactions require energy to activate the enzyme e. Enzyme-catalyzed reactions release more free energy than noncatalyzed reactions The following questions are based on the reaction A+B C+D Free Energy- A+B Progress of the Reaction C.D26. AN ORGANIC COFACTOR IN A CONJUGATED ENZYME?A. NEITHER VITAMINS NOR MINERALSB. MINERALS C. VITAMINSD. BOTH VITAMINS AND MINERALS 27. WHICH OF THE FOLLOWING STATEMENTS CONCERNING COFACTORS IS INCORRECT?A. VITAMINS CANNOT BE COFACTORSB. SOME COFACTORS ARE METAL IONS C. COFACTOR IS THE NON-PROTEIN PORTION OF ENZYME D. CONJUGATED ENZYMES CONTAIN COFACTORS 28. WHICH OF THE FOLLOWING VITAMINS IS NOT A FAT SOLUBLE VITAMIN? A. VITAMIN E B. VITAMIN C C. VITAMIN A D. VITAMIN D 29. WHAT HAPPENS TO SUBSTRATE MOLECULES AT AN ENZYME ACTIVE SITE?A. ALWAYS REACT WITH OXYGEN B. UNDERGO CHANGE TO A DESIRED PRODUCTS C. BECOME CATALYST C ALWAYS REACT WITH OXYGEN B UNDERGO CHANGE TO A DESIRED PRODUCT D. BECOME COVALENTLY BONDED TO THE ENZYME D. BECOME COVALENTLY BONDED TO THE ENZYME 30. ACTION OF CHEMICAL WARFARE AND ORGANOPHOSPHATE INSECTICIDE ARE BASED ON?A. REVERSIBLE ENZYME INHIBITOR B. IRREVERSIBLE ENZYME INHIBITOR C. REVERSIBLE ENZYME ACTIVATOR D. IRREVERSIBLE ENZYME ACTIVATOR
- 8. An enzyme has the ability to catalyze reactions of several unrelated compounds. The mechanism of how this enzyme operates is best explained by Oa. the lock-and-key theory Ob. the induced-fit theory Oc. the enzyme-substrate complex Od. the efficiency of the enzyme3. Which of the following statements regarding enzymes and transition states is true? a. stabilization of the transition state must be less than stabilization of ES for catalysis to occur b. binding of substrate to an enzyme often causes strain, thus promoting transition state formation c. the transition state conformation of an enzyme catalyzed reaction is identical to the conformation seen in the uncatalyzed transition state d. formation of the transition state always assures that the reaction will proceed to product e. none of the above are true 4. What is the starting point for selection of a suitable ion-exchange chromatography matrix for purification of a recombinant protein? a. Prediction of isoelectric point (pl) from the amino acid sequence. b. Test protein binding to an ion-exchange matrix at a range of pHs and salt concentrations. c. Test protein binding to a selection of anion and cation exchange matrices. d. Pass your sample through a preparative column and elute with a…5. a. In the process of converting ADP to ATP, water is circle one: (REQUIRED / RELEASED) and therefore this is called a reaction type. Does this type of reaction require an enzyme? circle one: (NO/YES / sometimes ) This reaction circle one: (REQUIRES / RELEASES) energy! b. In the process of converting ATP to ADP, water is circle one: (REQUIRED / RELEASED) and therefore this is called a reaction type. Does this type of reaction require an enzyme? circle one: (NO/YES / sometimes) This reaction circle one: (REQUIRES / RELEASES) energy! c. A given molecule of ATP can be broken down to ADP + P close to 1500 times in a day ("ATP cycle"). This is occurring in order to for cellular work. List a few examples of cellular work. d. Where does the energy to make ATP come from? It doesn't make sense to use ATP to make ATP. The energy to make ATP comes from specifically these types of macromolecules: and
- _1. Enzymes make reactions go by the activation energy of the reaction. A. slower: raising B. faster: lowering C. faster: raising D. slower: lowering 2. What do you call the model which assumes a high degree of resemblance between the shape of the substrate & the geometry of the binding site on the enzyme? A. Van't Hoff C. Induced Fit D. Lock and Key B. Michaelis-Menten Which of the following does not belong to the group? A. Hydrolases B. Lipases 3. C. Lyases D. Transferases1.Based on the graph, which of the following could be used to increase the reaction rate beyond point C? O A. increase the amount of substrate O B. increase the temp O c. 'decrease enzyme concentration O D. add more water ---9. For the graph below, which arrow indicates the ACTIVATION ENERGY for an ENZYME CATYLIZED reaction? reactants e.g. CO, + H,O products H,CO, Reaction coordinate O A B. C. Energy