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1. Currently, Sanger dideoxy sequencing invariably makes use of a thermal cycler (PCR machine) and heat stable DNA polymerase. As with PCR, the primer, deoxynucleoside triphosphates (with one ddNTP included per reaction), template DNA, and the heat stable DNA polymerase are in the reaction mixtures. The temperature is ramped to 95 °C, then cooled to 42 °C, then increased to 72 *C; this temperature sequence is repeated 30 times. a. What is the purpose of repeating the sequence many times? b. This method is often and inappropriately called "PCR sequencing". Why is it inappropriate to label this method as "polymerase chain reaction" sequencing?

1. Currently, Sanger dideoxy sequencing invariably makes use of a thermal cycler (PCR machine) and heat stable DNA polymerase. As with PCR, the primer, deoxynucleoside triphosphates (with one ddNTP included per reaction), template DNA, and the heat stable DNA polymerase are in the reaction mixtures. The temperature is ramped to 95 °C, then cooled to 42 °C, then increased to 72 *C; this temperature sequence is repeated 30 times. a. What is the purpose of repeating the sequence many times? b. This method is often and inappropriately called "PCR sequencing". Why is it inappropriate to label this method as "polymerase chain reaction" sequencing?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. Currently, Sanger dideoxy sequencing invariably makes use of a thermal cycler (PCR machine) and heat stable DNA polymerase. As with PCR, the primer, deoxynucleoside triphosphates (with one ddNTP included per reaction), template DNA, and the heat stable DNA polymerase are in the reaction mixtures. The temperature is ramped to 95 °C, then cooled to 42 °C, then increased to 72 *C; this temperature sequence is repeated 30 times. a. What is the purpose of repeating the sequence many times? b. This method is often and inappropriately called "PCR sequencing". Why is it inappropriate to label this method as "polymerase chain reaction" sequencing?
Transcribed Image Text:1. Currently, Sanger dideoxy sequencing invariably makes use of a thermal cycler (PCR machine) and heat stable DNA polymerase. As with PCR, the primer, deoxynucleoside triphosphates (with one ddNTP included per reaction), template DNA, and the heat stable DNA polymerase are in the reaction mixtures. The temperature is ramped to 95 °C, then cooled to 42 °C, then increased to 72 *C; this temperature sequence is repeated 30 times. a. What is the purpose of repeating the sequence many times? b. This method is often and inappropriately called "PCR sequencing". Why is it inappropriate to label this method as "polymerase chain reaction" sequencing?
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