1. Calculate the pH of a 1 L solution containing 6 mL of 1.5 M acetic acid and 5 mL of0.4 M sodium acetate. The pKa of acetic acid is 4.76.2. What is the ratio of conjugate base to acid of buffer (pKa=4.8) with pH=4.8?Show your work.3. Phosphate buffers are common in biochemistry lab. They can be made fromphosphoric acid (H;PO4):pКа-2.15pKa=7.20pKa=12.35H,PO,H,PO,HPO, 2 PO,*Which acid/conjugate base would you use to prepare a buffer at pH=8.11?Which acid/conjugate base would you use to prepare a buffer at pH=11.11?Which acid/conjugate base would you use to prepare a buffer at pH=3.11?4. Sulfhydryl groups are capable of ionizing (losing a proton). The pka of the sidechain of Cys is 8.18. Explain why Cys is drawn as -SH and not -S at physiologicalpH.5. Which of the amino acids below would most likely be found on the surface of aprotein? Which would be found on the inside of a globular protein? Explain youranswer.Asp, Phe, Ser, Leu, Arg 6. If amino acid X has pl=4.6, what is its charge in each of the following buffers?A. pH=10.212. pH=7.4C. pH=5D. pH=2.57. The figure below is the amino acid sequence of a growing peptide.NH2a. What is the 3rd amino acid?b. What level of protein structure does the figure represent?8. A Lys residue in a protein was changed to Asn, Asp, and Arg. Which substitution wouldhave the greatest effect on the tertiary structure? The least effect? Explain your answer.9. Histones are basic proteins that interact with DNA. What amino acids would be found inhigh abundance in histones? What type of interaction forms between histones and DNA?10. Receptor proteins are located on the surfaces of cells. They bind molecules or ligandsthat cannot directly enter the cell. The binding of a ligand to a receptor leads to somechange within the cell. In a receptor protein, a Glu is changed to Ala and binding of theligand decreases 100-fold. What does this tell you about the interaction between the ligandand receptor?

According to Henderson- hasselbalch equatlon.pH=pKa+log[salt ][acid]..........1stGiven,pKa=4.766ml…

Answered: 1. Calculate the pH of a 1 L solution…

1. Calculate the pH of a 1 L solution containing 6 mL of 1.5 M acetic acid and 5 ml of 0.4 M sodium acetate. The pKa of acetic acid is 4.76.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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