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1. Below is a partial sequence of a guide RNA. The underlined section of the RNA is designed to match a specific target DNA sequence. 5'-GGCGGAGCGGUUCUUGGCAGGUUUUAGAGCUAGAAAUAGC-3' View the partial gene sequence shown below. It contains a target DNA sequence that matches the guide RNA above. Circle the one PAM sequence in the top (5'-to-3') strand that is next to (-) this target DNA sequence. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG-3' 3'-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC-5' 2. From the sequence above, write down the guide RNA sequence that binds to the DNA, and the DNA sequence that it binds to (the complement of the target DNA). Label the 5' and 3' ends for both the RNA and DNA strands

1. Below is a partial sequence of a guide RNA. The underlined section of the RNA is designed to match a specific target DNA sequence. 5'-GGCGGAGCGGUUCUUGGCAGGUUUUAGAGCUAGAAAUAGC-3' View the partial gene sequence shown below. It contains a target DNA sequence that matches the guide RNA above. Circle the one PAM sequence in the top (5'-to-3') strand that is next to (-) this target DNA sequence. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG-3' 3'-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC-5' 2. From the sequence above, write down the guide RNA sequence that binds to the DNA, and the DNA sequence that it binds to (the complement of the target DNA). Label the 5' and 3' ends for both the RNA and DNA strands

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. Below is a partial sequence of a guide RNA. The underlined section of the RNA is designed to match a specific target DNA sequence. 5'-GGCGGAGCGGUUCUUGGCAGGUUUUAGAGCUAGAAAUAGC-3' View the partial gene sequence shown below. It contains a target DNA sequence that matches the guide RNA above. Circle the one PAM sequence in the top (5'-to-3') strand that is next to (-) this target DNA sequence. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG-3' 3'-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC-5' 2. From the sequence above, write down the guide RNA sequence that binds to the DNA, and the DNA sequence that it binds to (the complement of the target DNA). Label the 5' and 3' ends for both the RNA and DNA strands
Transcribed Image Text:1. Below is a partial sequence of a guide RNA. The underlined section of the RNA is designed to match a specific target DNA sequence. 5'-GGCGGAGCGGUUCUUGGCAGGUUUUAGAGCUAGAAAUAGC-3' View the partial gene sequence shown below. It contains a target DNA sequence that matches the guide RNA above. Circle the one PAM sequence in the top (5'-to-3') strand that is next to (-) this target DNA sequence. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG-3' 3'-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC-5' 2. From the sequence above, write down the guide RNA sequence that binds to the DNA, and the DNA sequence that it binds to (the complement of the target DNA). Label the 5' and 3' ends for both the RNA and DNA strands
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