1. A strand of dsDNA contains 22,008 bp. When relaxed and without strain, what is the linking number (Lo)? 2. The above dsDNA actually has an observed linking number, L, of 2000. a. What is the superhelix density? b. Is this dsDNA underwound or overwound? Explain. c. Provide three topoisomers of this dsDNA molecule.

1. A strand of dsDNA contains 22,008 bp. When relaxed and without strain, what is the linking number (Lo)? 2. The above dsDNA actually has an observed linking number, L, of 2000. a. What is the superhelix density? b. Is this dsDNA underwound or overwound? Explain. c. Provide three topoisomers of this dsDNA molecule.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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6.Rank order the following 10-base dsDNA sequences by "melting" temperature to single strandedness, the most heat stabile first. (You also have the option to choose any having equal melting temperatures.). MAKE SURE YOUR SEQUENCES DISPLAY IN A NON-PROPORTIONAL FONT SUCH AS 'COURIER' i.e., the two strands of each should line up "pairwise." А. 5'AAAAAAАAAАЗ' 3'TTTTTTTTTT5' B. 5'GGGGGGGGGG3' 3'СССССССССС5 C. 5'GGGGGAGGGG3' 3СССССТСССС5 D. 5'GGGGGGGGGG3' 3'ССССССGGCS'
2. A. B. C. G=% A = % T= % 6 In the bacteriophage DX174, the DNA is approximately 26% A. C= % G= % T = % Sea urchin DNA is approximately 18% G. C = % A = % T = % 6 17 19 10 11 The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. 12 13 Go Ent Into
Shown below are eight DNA sequences from different individuals. Q.Give the haplotype of each sequence by listing the specific bases at each variable position in that particular haplotype.
a. What are the linkage distances between m and r, between r and t, and between m and t?b. Determine the linkage order for the three genes.?c. What is the coefficient of coincidence (see Chapter 4) in this cross? What does it signify?
2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G = % % A = % In the bacteriophage DX174, the DNA is approximately 26% A. C = % G = % A = T= Sea urchin DNA is approximately 18% G. C = % T = % T= % % 64
1. Which of the following are generated due to the twisting of dsDNA? a. Hydrophobic bonds b. Van der Waal forces c. Hydrogen bonds d. Grooves 2. Which of the following terms refers to the annealing of ssDNA to complementary RNA? a. Homologous combination b. Hybridization c. Denaturation d. Telomere lengthening 3. Which of the following completes this statement? The melting temperature of DNA is the temperature at which__________. a. DNA denatures b. DNA bonds are reinforced c. Solid DNA becomes liquid d. Liquid DNA coils
3) Erwin Chargaff is considered one of the pioneering scientists in the field of molecular biology. In one of his studies, Chargaff observed that a sample of human DNA contained approximately 30% adenine and 20% guanine. Based on DNA structure, what are the expected percentages of the other nitrogenous bases in the sample? A. 10% thymine, 40% cytosine B. 20% thymine, 30% cytosine C. 30% thymine, 20% cytosine D. 40% thymine, 10% cytosine
35. M Heinz Shuster collected the following data on the base composition Move ANALYSIS ribgrass mosaic virus (H. Shuster, in The Nucleic Acids: Chemistry and Biology, vol. 3, E. Chargaff and J. N. Davidson, Eds. New York: Academic Press, 1955). On the basis of this information, is the hereditary information of the ribgrass mosaic virus RNA or DNA? Is it likely to be single stranded or double stranded? Percentage A T U Ribgrass 29.3 25.8 18.0 0.0 27.0 mosaic virus Leibniz Institute for Age Research, Fritz Lipmann-Institute. Ribgrass mosaic virus.
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