1) Use the plot of reaction rate as a function of substrate concentration for an enzyme-catalyzed reaction below(left) and its Lineweaver-Burk transformation (right) to answer the following questions. (Please ignore the axis values on the Lineweaver-Burk plot; they are not needed to answer the questions.) cs Scanned with a) Appropriately label the axes on each of the plots above and indicate which of the curves above represent the enzyme's activity when: iit is uninhibited ii) it is in the presence oflow, moderate, and high concentrations of inhibitor (be sure to clearly indicate which curve is which)? b) On the plots above, show how you could determine each ofthe following parameters iVmax for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration ii) KM for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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1) Use the plot of reaction rate as a function of substrate concentration for an enzyme-catalyzed
reaction below(left) and its Lineweaver-Burk transformation (right) to answer the following
questions. (Please ignore the axis values on the Lineweaver-Burk plot; they are not needed to
answer the questions.)
cs Scanned with
a) Appropriately label the axes on each of the plots above and indicate which of the curves above
represent the enzyme's activity when:
iit is uninhibited
ii) it is in the presence oflow, moderate, and high concentrations of inhibitor (be sure to clearly
indicate which curve is which)?
b) On the plots above, show how you could determine each ofthe following parameters
iVmax for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration
ii) KM for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration
Transcribed Image Text:1) Use the plot of reaction rate as a function of substrate concentration for an enzyme-catalyzed reaction below(left) and its Lineweaver-Burk transformation (right) to answer the following questions. (Please ignore the axis values on the Lineweaver-Burk plot; they are not needed to answer the questions.) cs Scanned with a) Appropriately label the axes on each of the plots above and indicate which of the curves above represent the enzyme's activity when: iit is uninhibited ii) it is in the presence oflow, moderate, and high concentrations of inhibitor (be sure to clearly indicate which curve is which)? b) On the plots above, show how you could determine each ofthe following parameters iVmax for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration ii) KM for the uninhibited enzyme and for the enzyme under the highest inhibitor concentration
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