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1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: &=3600 M¹ cm ¹ (molar absorption coefficient for the product dopachrome) v=0.004 L (final volume in the cuvette) time (min) 0 0.5 1 1.5 2 2.5 3 A at 475 nm 0.1091 10.219 0.3186 0.4106 0.4956 0.5724 0.6451 b) If the tyrosinase concentration in the medium is 0.2 mg/mL, find the specific enzyme activity.

1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: &=3600 M¹ cm ¹ (molar absorption coefficient for the product dopachrome) v=0.004 L (final volume in the cuvette) time (min) 0 0.5 1 1.5 2 2.5 3 A at 475 nm 0.1091 10.219 0.3186 0.4106 0.4956 0.5724 0.6451 b) If the tyrosinase concentration in the medium is 0.2 mg/mL, find the specific enzyme activity.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: E = 3600 M ' cm (molar absorption coefficient for the product dopachrome) v = 0.004 L (final volume in the cuvette) time (min) A at 475 nm 0.1091 0.5 10.219 1 0.3186 1.5 0.4106 0.4956 2.5 0.5724 3 0.6451 b) If the tyrosinase concentration in the medium is 0.2 mg/mL, find the specific enzyme activity.
Transcribed Image Text:1) Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: E = 3600 M ' cm (molar absorption coefficient for the product dopachrome) v = 0.004 L (final volume in the cuvette) time (min) A at 475 nm 0.1091 0.5 10.219 1 0.3186 1.5 0.4106 0.4956 2.5 0.5724 3 0.6451 b) If the tyrosinase concentration in the medium is 0.2 mg/mL, find the specific enzyme activity.
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