1) Perform the necessary calculations and fill in the values in the table below (be sure to include units). Submit or attach your work.
1) Perform the necessary calculations and fill in the values in the table below (be sure to include units). Submit or attach your work.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Need help filling in the table from the data provided.
Transcribed Image Text:1) Perform the necessary calculations and fill in the values in the table below (be sure to include units).
Submit or attach your work.
Kapp
M
Vapp
max
Turnover
Ki
Uninhibited
100 μM Na3PO4
500 μM Na3PO4 1 mM L-Phe
5 mM L-Phe
![1/Vo
20
18
16
14
12
10
8
6
4
NO
2
0
1/Vo
25
20
15
10
5
0
0.005
Substrate No Inhibi 100uM Na 500uM Na3PO4
0.04 4.06409 7.591549 20.63158
0.02 3.097701 4.656587 11.62264
0.01 2.626066 3.376664 6.654321
0.005 2.317034 2.479586 4.091082
0.003333 2.213552 2.45 3.246988
0
Lineweaver-Burk Comparison L-Phe
0.01
0.005
No Inhibitor
Linear (No Inhibitor)......
0.015
0.01
No Inhibitor
......... Linear (No Inhibitor)
0.02
0.015
1/[S]
10 mM L-Phe
Linear (10 mM L-Phe)
0.025
0.02
1/[S]
V.32.079x+15.777..
Lineweaver-Burk Comparison Na3PO4
0.025
V=31.305x+5.2731
49.973x+2:0808-
0.03
100 UM Na3PO4
......... Linear (100 UM Na3PO4)
0.035
Substrate Rate noni 10 IPhe
50 mM L-Phe
Linear (50 mM L-Phe)
50LPhe
0.04 4.06409 6.42623 17.3871
0.02 3.097701 6.107649
15.68
0.01 2.626066 5.585492 16.08955
0.005 2.317034 5.528205 16.14981
0.003333 2.213552 5.170264 16.08955
y = 474.21x +1.82.
0.03
y=142.05x +.1.8855
0.04
R²0:9983.
0.035
0.04
0.045
0.045
100 UM Na3PO4
Linear (100 UM Na3PO4)
Rate (UM/s)
0.5
0.4
0.3
0.2
0.1
0
Rate (UM/s)
0
0.5
0.4
0.3
0.2
0.1
0
0
Michaelis Menten Comparison Na3PO4
●
50
●
No Inhibitor
•
•
●
100
50
150
200
Substrate Concentration (UM)
Michaelis Menten Comparison L-Phe
100
100 UM Na3PO4 • 500 UM Na3PO4
250
150
200
250
Substrate Concentration (UM)
300
●No Inhibitor • 1 mM L-Phe * 5 mM L-Phe
300
350
350](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff800a840-0309-4834-92a3-4244363b7afa%2Ff1ae4918-6f56-4211-b54f-92010db5ab46%2Ffpnj87g_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1/Vo
20
18
16
14
12
10
8
6
4
NO
2
0
1/Vo
25
20
15
10
5
0
0.005
Substrate No Inhibi 100uM Na 500uM Na3PO4
0.04 4.06409 7.591549 20.63158
0.02 3.097701 4.656587 11.62264
0.01 2.626066 3.376664 6.654321
0.005 2.317034 2.479586 4.091082
0.003333 2.213552 2.45 3.246988
0
Lineweaver-Burk Comparison L-Phe
0.01
0.005
No Inhibitor
Linear (No Inhibitor)......
0.015
0.01
No Inhibitor
......... Linear (No Inhibitor)
0.02
0.015
1/[S]
10 mM L-Phe
Linear (10 mM L-Phe)
0.025
0.02
1/[S]
V.32.079x+15.777..
Lineweaver-Burk Comparison Na3PO4
0.025
V=31.305x+5.2731
49.973x+2:0808-
0.03
100 UM Na3PO4
......... Linear (100 UM Na3PO4)
0.035
Substrate Rate noni 10 IPhe
50 mM L-Phe
Linear (50 mM L-Phe)
50LPhe
0.04 4.06409 6.42623 17.3871
0.02 3.097701 6.107649
15.68
0.01 2.626066 5.585492 16.08955
0.005 2.317034 5.528205 16.14981
0.003333 2.213552 5.170264 16.08955
y = 474.21x +1.82.
0.03
y=142.05x +.1.8855
0.04
R²0:9983.
0.035
0.04
0.045
0.045
100 UM Na3PO4
Linear (100 UM Na3PO4)
Rate (UM/s)
0.5
0.4
0.3
0.2
0.1
0
Rate (UM/s)
0
0.5
0.4
0.3
0.2
0.1
0
0
Michaelis Menten Comparison Na3PO4
●
50
●
No Inhibitor
•
•
●
100
50
150
200
Substrate Concentration (UM)
Michaelis Menten Comparison L-Phe
100
100 UM Na3PO4 • 500 UM Na3PO4
250
150
200
250
Substrate Concentration (UM)
300
●No Inhibitor • 1 mM L-Phe * 5 mM L-Phe
300
350
350
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