1 Esters can be used as solvents in chromatography. The following shows a chromatogram of plant acids. solvent front the cross represents the centre of the spot baseline sample 1 sample An ester was used as the solvent and the chromatogram was sprayed with bromothymol blue. (1) Suggest why it was necessary to spray the chromatogram. (ii) Explain what is meant by the Rf value of a sample. (iii) Calculate the Rf values of the two samples and use the data in the table to identify the plant acids.

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Introduction to chemistry

Chemistry is a branch of science that deals with the analysis of material and the substances that comprise it. It also manages the properties of these substances and their reactions to form new substances. It is basically centered on particles and atoms that make up components and mixtures. These chemical species will in general connect using bonds.